制取小麦旗叶粗酶液的最佳缓冲体系研究.pdfVIP

陈玉串生 陈玉串生 安徽农业科学，Joumal of Anhui Agri. Sci.2016 ,44(35) : 17 -19 责任编辑 责任校对 制取小麦旗叶粗酶液的最佳缓冲体系研究 高玲 (青岛农业大学生命科学学院.山东青岛 266109) 摘要 [ 目的]探讨制取小麦毒草叶粗略液的最佳缓冲体系。 [方法] 采用不同的缓冲体系制备小麦旗叶粮酶液，测定小麦旗叶中过氧化 物酶(POD) 、淀粉麟、苯丙氨酸解氨嗨(PAL) 、脂氧合麟(1βX) 的活力。 [结果} 当 pH 为 6.2 时，POD 活力最高;当 pH 为 5.8 时，淀粉酶 活力最高;当 pH 为 7.0 时，PAL 和1βX 活力最高。 选用磷酸氢二锅，磷酸二氢纳缓冲液为提取缓冲液时.PAL 和IρX 活力最高。 当此 生在冲液浓度为 0.15 moνL 时，POD 活力最高;当此缓冲液浓度为0.10 mol/L 时，淀粉峰和IρX 活力最高;当此缓冲液浓度为0.05 mol/L 时.PAL 活力最高。 [结论]制取粗略泼的最佳缓冲体系是pH 7 . 0 ,0.10 mol/L 的磷酸氢二钩，磷酸二氢钩缓冲淡。 关键词 小麦;旗叶;粗鳞泼;缓冲体系 中图分类号 S512.1 ;Q55 文献标i只码 A 文章编号 0517 -6611(2016)35 -∞17 - 03 Study 0 0 the Optimum BufIeriog System for Prepariog Crud e Enzymes io F 1ag Leaves of WI1eat GAO Ling ( College of Life Sciences. Qingdao Agricultural University , Qingdao , Shandong 2661ω) Abstract [ Objective] To study the optimum buffering system for preparing crude enzymes in f1ag 1eaves of wheat. [ Me由od ] Taking flag leaves of wheat as test materia1 s. different buffering systems were used to prepare crude enzymes in flag leaves of wheat. And the activities of POD , amylase , PAL , LOX in flag leaves of wheat were determined. [Result] When pH was 6.2 , POD activity w幽 the highest; When pH was 5. 8. amylase activity was the highest; When pH was 7. 0 , both PAL activity and LOX activity reached the maximum values. When Na 日PO.-NaH PO. buffer was used as the extracting buffer in the test. PAL and 1βX activities both reached the maximum va1ues. When the 2 2 concentration of NazHPO. -NaHzPO. bufIer was 0.15 mo