浓硫酸与温水浸种对决明子萌发的影响研究.pdfVIP

第24 卷 第1 期 天 津 农 学 院 学 报 Vol. 24 ，No. 1 2017 年3 月 Journal of Tianjin Agricultural University March ，20 17 文章编号：1008-5394 （2017 ）0 1-0005-03 浓硫酸与温水浸种对决明子萌发的影响研究 王景仪，孙宁通信作者 ，王妍，杨文琪，潘志达 （天津农学院农学与资源环境学院，天津 300384 ） 摘 要：决明子是具较高医疗养生价值的 中草药之一，属于硬实种子，因发芽率低导致其产量也较 低。本文利用温水和不同浓度的浓硫酸浸种处理对种皮进行软化 ，发芽后检测决明子的发芽势、发 芽率、叶绿素含量、游离脯氨酸含量等指标。结果表明：决明子在 80%浓硫酸 中浸种 20 min 或 60 ℃ 温水浸种 2 h 处理下，发芽率均达 95%以上，并可明显的促进幼苗的初期生长。 关键词：决明子；种皮软化；发芽率；幼苗 中图分类号：S567.239 文献标识码：A Effects of Concentrated Sulfuric Acid and Warm Water Soaking on the Seeds Germination of Cassia obtusifolia WANG Jing-yi, SUN NingCorrespongding Author, WANG Yan, YANG Wen-qi, PAN Zhi-da （College of Agronomy and Resource Environment, Tianjin Agricultural University, Tianjin 300384, China ） Abstract: Cassia obtusifolia is one of the Chinese herbal medicines with high value of medical health. The seed of C. obtusifolia is belonging to hard seeds, which results in the lower germination rate while the low yield. Warm water and different concentrated H SO were used to soften seed-coat; and the indexes, such as its seeds’ germination potential, germination rate, 2 4 chlorophyll content, free proline content, etc. were measured after the seeds germinating. The results showed that the seed germination rate was up to 95% under the soaking treatment of 80% H SO with 20 min or 60 ℃ water with 2 h, and it could 2 4 obviously promote the initial growth of seedlings. Key words: Cassia obtusifolia L