麦纤散对UC大鼠结肠组织ERK12 mRNA表达的影响.pdfVIP

江西 中医药大学学报2016年8月第28卷第4期 JOURNALOFJIANGXIUNIVERSITYOFTCM 2016Vo1．28No．4 p 江中医药大学基础医学院 杭州 310053) r (extracellularregulatedproteinkinases1，ERKI)、细胞外信 f _A表达的影响，探讨其作用机制。方法：将实验动物分为6 模，造模成功后 ，分别连续灌胃给药2周观察大鼠一般情 P [K1、ERK2mRNA表达水平。结果：麦纤散能明显改善 UC 结肠组织中ERK1mRNA显著增加 (P0．05)，美沙拉嗪 散可改善UC大鼠一般情况，其作用机制可能与上调结肠 e l o 2mRNA in ColonoftheRatswithUlcera． i LILi—le，SHUINan—nan，XU Fang—li，SHIHui—qin，ZHENG Hong—bin g ThebasicschoolofmedicineaffiliatedtoZhejiangChineseMedicinalUniversity，Hangzhou，310053，china． Abstract：Objective’：ToobservetheinfluenceofmaixiansanonexpressionsofERK1geneandERK2geneincolonoftheratswithul— cerativecolitis(UC)，andtoidentifyitsunderlyingmechanism．Methods：Experimentalanimalsweredividedintosixgroupsandtrea— s tedwithTNBStoinduceulcerativecolitis．Aftersuccessfulmodeling，eachexperimentalgroupwasadministratedNS，mesalazine，and P Maixiansanrespectivelywithtwoweeks．andthegeneralsituationwasobservedand．Thecolontissuewascollected24hourslaterafter thelastadministration，andtheexpressionsofERK1geneandERK2geneincolontissuewasdetectedbymeansofRT—PCR．Re— suits：Maixiansancanobviouslyimproveth