脱铁乳铁蛋白铬络合物制备方法研究_吴扬.pdf

76 2009, Vol. 30, No. 14 食品科学 ※工艺技术 脱铁乳铁蛋白铬络合物制备方法的研究 吴 扬，胡志和* ，杨柏标 (天津市食品生物技术重点实验室，天津商业大学生物技术与食品科学学院，天津 300 134) 摘 要：以乳铁蛋白(lactoferrin ，LF)和六水三氯化铬为主要原料制备脱铁乳铁蛋白铬，确定脱铁乳铁蛋白与Cr3+ 络 合的条件，并对络合物采用红外吸收光谱进行表征。结果表明：乳铁蛋白经醋酸调pH 值到2 .25 或经柠檬酸调pH 值 到2 .50 后，均能使F e3+ 游离出来；脱铁乳铁蛋白与Cr 3+ 络合的条件为：温度60 ～70 ℃，p H7 .0 ～7 .6 ，脱铁乳铁 蛋白与六水三氯化铬物质的量的浓度比1:2 ，搅拌时间2h 以上。 关键词：乳铁蛋白；三氯化铬；脱铁乳铁蛋白铬；红外吸收光谱 Preparation of Apotransferrin-Chromium Complex WU Yang ，HU Zhi-he* ，YANG Bo-biao (Tianj in Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianj in University of Commerce, Tianj in 300 134, China) Abstract ：Apotransferrin was prepared from lactoferrin u sing acetic acid or citric acid, and the apotransferrin prepared using acetic acid was employed to prepare apotran sferrin-chromium complex . The effects of acetic acid and citric acid on the apotransferrin preparation were compared by determining product yield and analyzing chemical structure by means of UV and IR absorption spectroscopy. The preparation conditions of apotransferrin-chromium complex were investigated by one-factor- at-a-time experiments, and the product was characterized. Fe3+ could be effectively dissociated from lactoferrin by adjusting pH to 2.25 u sing acetic acid or to 2.50 using citric acid. Compared to citric acid, the use of acetic acid yielded more apotransferrin, but the apotran sferrin products prepared using the two reagents had the same functional groups. The optimal complex condi- tions of apotransferrin with Cr3+ were as follows: 60 －70 ℃, pH 7.0 －7.6, molar ratio of apolactoferrin to chromium trichloride 1:2, and stirr