拮抗链霉菌F10对油茶炭疽病菌抑菌作用研究.PDF

拮抗链霉菌F10 对油茶炭疽病菌抑菌作用研 究 1，2 1 1 1 1 1 宋光桃 , 赵莹，周国英，李河，王慧敏，游牧 (1.中南林业科技大学，长沙410004；2.湖南环境生物职业技术学院，衡阳421005) 摘 要：拮抗链霉菌菌株 F10 对油茶炭疽病菌有良好的拮抗效果。为了进一步开发利用菌株 F10，以油茶炭疽病菌为指标，研究了菌株F10 对油茶炭疽病菌的抑菌作用。结果表明，菌 株F10 能影响菌丝正常生长和分生孢子的萌发，菌丝出现节间变短、变粗、扭曲变形、菌丝 严重交联、细胞质聚集、外溢等现象；能分泌蛋白酶和纤维素酶影响油茶炭疽病菌的细胞壁； 并对水稻、蚕豆、白菜种子的萌发有促生作用。 关键词：拮抗链霉菌F10；油茶炭疽病菌；抑菌作用 Study on the Inhibitory Mechanism of Antagonistic Streptomyces F10 to Colletotrichum gloeosporioide 1,2 1 1 1 SONG Guang-tao ,ZHAO Yi, ZHOU Guo-ying , LI He , WANG Huiming,YOU Mu 1 ( Central South University of Forestry and Technology, Changsha,410004; 2Hunan Environment-Biologic Polytechnic, Hengyang 421005) Abstract: Antagonistic streptomyces strain F10 has good antagonistic activity to Colletotrichum gloeosporioides . In order to development and utilization strain F10, made antimicrobial activity for C. gloeosporioides as the indicator, we studied the inhibitory mechanism of strain F10 resistance to C. gloeosporioides . The results showed that the strain F10 could affect the growth of mycelia and conidia germination of C. gloeosporioides, resulting into shortened and thick internode, twist deformation, serious cross linking of mycelia, cytoplasmic aggregation and the leak of cytoplasm. the strain F10 could secrete protease and cellulose to affect cell wall of C. gloeosporioides, and which could promote seed germination of rice,broad bean and Chinese cabbage. Key words: Antagonistic streptomyces F10; C. gloeosporioides; Inhibitory mechanism 拮抗微生物的抑菌机理主要有产生拮抗物质、重寄生作用、交叉保护作用、竟争作用和 诱导植物产生抗病性等。阐明拮抗微生物的抑菌机理，对了解拮抗微生物的特性和病害的致 病原因，以及确定拮抗微生物能否可在生产实践中应用