醋马钱子炮制工艺研究.docVIP

醋马钱子的炮制工艺研究 　　摘要：目的规范并优化醋马钱子的炮制工艺，获得最佳工艺。方法指标的评价以士的宁和马钱子碱两种生物碱的含量作为标准，通过对醋马钱子炮制工艺中的重点环节进行考察，优选关键因素进行正交试验并结合单因素优化试验，解决步骤的简化和有效生物碱尽量保留、毒性生物碱尽量降低的问题，得出最佳炮制工艺。结果通过对醋马钱子炮制工艺中重点环节的考察，优选出浸泡时间、醋液浓度和醋煮时间三个因素进行正交试验。结果显示，A3B2C1为醋马钱子最佳炮制工艺，即浸泡天数5d，白醋浓度2.5%，醋煮时间1h。其中，影响醋马钱子炮制工艺中因素的主次顺序为：醋煮时间水浸天数醋液浓度。结论该方法为大规模生产提供了理论基础和依据 关键词：醋马钱子；炮制工艺；正交试验；单因素优化试验 中图分类号：R283文献标志码：A文章编号：1007-2349（2016）10-0066-04 Study on Processing Technology of Vinegar-soaked Strychnos Nux Vomica ZENG Pei-yu1， GAO Ming1， HUANG Yu-mei2， WU Zhi-jian2， MAI Min-xin2 JIANG Li-yun1， MA En-yao1， ZHU Qi-Liang1 （1. Guangzhou University of Traditional Chinese Medicine， Guangzhou 510006， Guangdong； 2.Chinese Medicine Pieces Factory， Guangzhou Medicinal Material Corporation， Guangzhou 510360，Guangdong） 【Abstract】Objective： To standardize and optimize the processing technology of vinegar-soaked Strychnos Nux Vomica to obtain the best technology. Methods： Indicative evaluation was made by taking content of strychnine and brucine as a standard. Through the inspection of key links in vinegar-soaked Strychnos processing technology， key factors were preferably chosen to conduct an orthogonal experiment and a single factor optimization test to simplify its procedure， preserve its effective alkaloids and minimize its toxic alkaloids so as to obtain the best processing technology. Results： Through the study of vinegar-soaked Strychnos processing technology， best soaking time， concentration of vinegar and vinegar-cooking time were preferably used to conduct the orthogonal test. The results showed that A3B2C1 was the best processing technology for vinegar-soaked Strychnos， i.e. 5-day soaking， 2.5% of vinegar concentration， one-hour vinegar-cooking time. Among them， the factors influencing vinegar Strychnos processing were orderly as， vinegar-cooking timesoaking daysvinegar concentration. Conclusion： The method provides a theoretical basis and foundation for mass production. 【Key words】vinegar-soaking Strychno