胭脂鱼Effect of mercury, cadmium, nickel, chromium and zinc on kinetic properties.pdfVIP

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胭脂鱼Effect of mercury, cadmium, nickel, chromium and zinc on kinetic properties.pdf

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胭脂鱼Effect of mercury, cadmium, nickel, chromium and zinc on kinetic properties

Toxicology in Vitro 21 (2007) 408–416 /locate/toxinvit EVect of mercury, cadmium, nickel, chromium and zinc on kinetic properties of NADPH-cytochrome P450 reductase puriWed from leaping mullet (Liza saliens) Azra Bozcaarmutlu a,¤, Emel Arinç b a Department of Chemistry, Institute of Natural and Applied Sciences, Abant Izzet Baysal University, 14280 Bolu, Turkey b Biochemistry Graduate Programme and Department of Biological Sciences, Middle East Technical University, Ankara, Turkey Received 17 April 2006; accepted 4 October 2006 Available online 10 October 2006 Abstract Information on the mechanism of metal ion inhibition of NADPH-cytochrome P450 reductase is limited. The purpose of the present paper was to elucidate in vitro eVect of Hg+2, Cd+2, Ni+2, Cr+3 and Zn+2 ions on the puriWed mullet NADPH-cytochrome P450 reductase. NADPH-cytochrome P450 reductase was puriWed from detergent-solubilized liver microsomes from leaping mullet (Liza saliens). All of the metal ions caused inhibition of the enzyme activity except Zn+2. At 50 M metal concentration, Hg+2 inhibited the cytochrome P450 reductase activity completely (100%), while, at the same concentrations, Cd+2, Cr+3 and Ni+2 caused 66%, 65% and 37% inhibition, respec- tively. At 50 M metal concentration, Zn+2 had no apparent eVect on cytochrome P450 reductase activity. The IC values of HgCl ,