附子中6种生物碱质谱裂解机制及醇解产物研究.pdfVIP

中国中医急症 2016 年 12 月第 25 卷第 12 期 JETCM. Dec. 2016 , VoJ. 25 , No.12 一2278- ·奥验报告· 附子中 6 种生物碱质谱裂解机制及 醇解产物研究* 张翅1.2 潘德林I.2未雅宁1.2 郝莉1 [1.华润三九(雅安)药业有限公司，四川雅安 625000;2.成都中医药大学，中药资源系统研 究与开发利用省部共建国家重点实验室培育基地，四川成都 611137 ] 中图分类号:R285.5 文献标志码:A 文章编号: 1ω4-745X(2016) 12-2278-05 doi: 10.3969/j.issn.lOO4-745X.2016.12.021 [摘要]目的探讨附子中 6 种生物碱裂解规律，研究双醋型生物碱乙醇解产物。方法采用 HPLC-ESI-MS 技术，在正离子模式下，扫描分析双醋型生物碱在乙醇的转化产物，并阐述其规律。结果通过对已知 6 种生 物碱的多级质谱分析及文献调研，总结了 6 种醋型生物碱的裂解规律，并确定了双醋型生物碱的乙醇产物。 结论 3 种双目旨型生物碱在乙醇中，发生乙醇解反应，分别生成 8-乙氧基新乌头碱、8-乙氧基乌头碱和 8-乙 氧基次乌头碱;由于反应在加热条件下进行，还有焦乌头碱的产生。 [关键词]附子乌头碱双醋型生物碱裂解规律醇解产物 Study on the Fragmentation Behavior and Alcoholysates of Aconitum Alkaloids in Aconitum Carmichaeli Debx ZHANG Chi ,PAN Delin ,ZHU Yaning, et 01. China Resources San}iu (Yαan) Pharmaceutico1 Co. ,Ltd. , Sichuan , Yaan 625000 , China. [Abstract] Objective: To investigate the pyrolysis mechanism of six alkaloids in Aconitum cannichaeli Debx , and identify their alcoholysates. Methods: HPLC-ESI-MS was used in this study. The first and multi-stage mass spectrum of aconitum alkaloids were obtained in positive ion mode. Results: Through the analysis of multi-stage mass spectromet巧， the fragmentation pattems of six alkaloids were illuminated , and the alcoholysates of the diester alkaloids were identified. Concl皿ion: 咀uee diester alkaloids are alcoholyzed in ethanol and generated three new products respectively ,8 -ethoxy -mesaconitine , 8 -ethoxy -aconitine and 8 -ethoxy -hypaconitine; pyraconitine is generated at the same time because the reaction proceeds under heating. [Key words] Aconitum carmichaeli Debx; Aconitine; Diester alkaloids; Fragmentation regularity; Alcoholysate 附子为毛在科植物乌头 (Aconitum carmichaeli MS 获得质谱数据并进行分析 推断出 6 种生物碱的 Debx.)子根的加工品，是临床常用的中药材，具有回阳