茶树中精氨酸脱羧酶基因的cDNA克隆和序列分析.PDFVIP

安徽农业大学学报, 2013, 40(3): 464-469 Journal of Anhui Agricultural University 网络出版时间：2013-4-26 15:36:36 [URL] /kcms/detail/34.1162.S1536.011.html 茶树中精氨酸脱羧酶基因的 cDNA 克隆及序列分析 * * 徐 乾，史成颖 ，宛晓春 ，汤志近 (安徽农业大学农业部茶树生物学与茶叶加工重点实验室，合肥 230036) 摘 要：通过 SMART RACE PCR 技术获得了茶树中精氨酸脱羧酶（ADC ）的 cDNA 全长序列，并登录 GenBank （登录号为JQ653274 ）。ADC 基因 cDNA 全长为 2 988 bp ，开放阅读框（ORF ）全长为2 163 bp ，共编码720 个氨 基酸，编码的蛋白质的分子量为 77.430 kDa ，理论等电点为5.373 ，其序列中不存在卷曲螺旋结构。ADC 蛋白为亲 水性非分泌不稳定蛋白，不跨膜运动，无信号肽序列存在，共有 41 个可能的磷酸化位点，存在于叶绿体基质中， 是细胞质蛋白。精氨酸脱羧酶的基因 cDNA 全长序列的获得，对于进一步研究精氨酸在茶树氮代谢中的作用及茶 氨酸代谢途径提供基础。 关键词：茶精氨酸脱羧酶；全长 cDNA；基因克隆；序列分析 中图分类号：S571.1 文献标识码：A 文章编号：1672−352X (2013)03−0464−06 Cloning and sequence analysis of arginine decarboxylase gene from Camellia sinensis XU Qian, SHI Cheng-ying, WAN Xiao-chun, TANG Zhi-jin (Key Laboratory of Tea Biology Processing of Ministry of Agriculture, Anhui Agricultural University, Hefei 230036) Abstract: The full-length cDNA of arginine decarboxylase (ADC , GenBank accession No. JQ65327) gene was obtained by SMART RACE PCR method. It is 2 988 bp in length with an open reading frame (ORF) of 2 163 bp, encoding 720 amino acids with deduced molecular weight of 77.430 kDa and theoretical pI value of 5.373. Bioin- formatics analysis show that ADC is a hydrophilic and unstable protein without any signal peptide, has no winded helix structure and transmembrane domain. There are 41 phosphorylation sites within the polypeptide chain. ADC is non-secreted protein and it functions in chloroplast stroma. The sequence analysis of ADC gene would bring some new clues for further exploration of the function of ADC in nitrogen metabolism and theanine biosynthesis. Key words: arginine decarboxylase; full-length cDNA; gene cloning; sequence analysis