蝉拟青霉LB菌株生物学特性.PDFVIP

微生物学通报 NOV 20, 2009, 36(11): 1670~1675 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 研究报告 蝉拟青霉LB 菌株的生物学特性 1,2* 1 3 3 2 李 忠 曾桂萍 邹 晓 刘爱英 金道超 (1. 550025) (2. 550025) (3. 550025) 摘 要: 本文研究碳源、氮源、温度、湿度、pH 值和光照等对蝉拟青霉LB 菌株生长、产孢和孢 子萌发的影响。结果表明, 适合该菌株菌落生长和产孢的最佳碳源是可溶性淀粉和蔗糖, 最佳氮 源为蛋白胨; 菌丝生长和孢子萌发的最适温度范围是 25°C~27°C, 产生分生孢子的最适温度是 25°C; 分生孢子萌发所需湿度范围是RH 90%~100%, 当RH 低于90%时很难萌发; 在pH 值4~10 的范围内该菌能生长和产孢, 菌丝生长最适pH 为6, 产生分生孢子和孢子萌发最适pH 范围为6~7; 光照处理对该菌产孢有一定的影响; 分生孢子的致死条件为55°C 10 min 。生物学特性显示, 蝉拟 青霉LB 菌株是一株对营养要求不高、对环境适应能力较强的昆虫病原真菌。 关键词: 蝉拟青霉, 生物学特性, 产孢量, 孢子萌发 Biological Characteristics of Paecilomyces cicadae LB Strain LI Zhong1,2* ZENG Gui-Ping1 ZOU Xiao3 LIU Ai-Ying3 JIN Dao-Chao2 (1. College of Agronomy, Guizhou University, Guiyang, Guizhou 550025, China) (2. Key Laboratory for Plant Pest Management in Mountain Agriculture, Guiyang, Guizhou 550025, China) (3. Institute of Fungus Resources, Guizhou University, Guiyang, Guizhou 550025, China) Abstract: The effect of carbon source, nitrogen source, temperature, moisture, pH and light on the fungal growth, conidia production and conidia germination of Paecilomyces cicadae LB were studied. For the fun- gal growth and conidia production, the optimum carbon sources were soluble starch and glucose, while the optimum nitrogen source was pe