中药天年饮对衰老大鼠睾丸功能的影响论文.docVIP

　　中药天年饮对衰老大鼠睾丸功能的影响论文 乔跃兵， 马秀艳， 张松岩， 薛景凤 【关键词】 天年饮；,,衰老；,,睾丸；,,睾酮；,,超氧物歧化酶 摘要：目的观察中药天年饮对衰老大鼠睾丸功能的影响。方法40只SD大鼠随机分为正常组、衰老模型组、天年饮用药组和阴性对照组，每组均为10只。采用D半乳糖连续腹腔注射制作亚急性衰老的大鼠模型。ELISA方法检测各组大鼠血清睾酮含量、黄嘌呤氧化酶法检测睾丸组织超氧化物歧化酶（superoxide dismutase,SOD）的活性、HE染色观察睾丸生精小管的形态结构。结果衰老大鼠血清睾酮含量、睾丸组织SOD活性明显下降（与正常大鼠相比较P 0.01）；衰老大鼠睾丸生精小管内生精细胞层数减少、细胞稀疏，生精小管管腔变狭窄。天年饮可明显抑制衰老大鼠血清睾酮含量和睾丸组织SOD活性的下降（用药组与模型组相比较P 0.01）；并能明显改善衰老大鼠睾丸生精小管的形态结构。结论 中药天年饮可明显改善衰老大鼠睾丸的功能，具有一定延缓衰老的作用。 关键词：天年饮； 衰老； 睾丸； 睾酮； 超氧物歧化酶 Effects of Traditional Chinese Medicine Tiannianyin on Testis Functions of Aging Rats Abstract：ObjectiveTo observe the effects of traditional Chinese medicine Tiannianyin on testis functions of aging rats. Methods40 SD rats : normal group, aging model group, Tiannianyin group, negative control group,.freelodel rats ade by injecting D-gal into abdominal cavity continuously. ELISA , xanthine oxidase method to detect the superoxide dismutase(SOD) activity in testis, HE staining to observe the morphology of seminiferous tubule. ResultsThe testosterone content in serum and the SOD activity in testis of aging rats decreased significantly pared al rats (P 0.01). The seminiferous tubule of aging rats appeared obviously morphological changes: the spermatogenic cells in seminiferous tubule reduced and arranged sparsely. Tiannianyin could inhibit the decreasing of testosterone content in serum and SOD activity in testis significantly (Tiannianyin group pared odel group: P 0.01), and significantly improve the morphology of seminiferous tubule.ConclusionTraditional Chinese medicine Tiannianyin can improve the testis functions of aging rats significantly, and has anti-aging effects. Key utase 人类的衰老与性腺结构和功能的退化密切相关。随着年龄的增长，睾丸的结构和功能会出现一个由盛到衰的演变过程.freelg/kg，1次/d, 共40 d) 制作亚急性衰老的大鼠模型。模型组大鼠于造模成功后不再作任何处理； 用药组大鼠于造模成功后每天用天年饮灌胃, 共30 d；阴性对照组大鼠在造模成功后每天灌胃同等剂量的生理盐水, 时间同用药组大鼠。 1.2 中药的组成及用量天年饮由何首乌、怀牛膝、肉苁蓉、淫羊藿、丹参、茯苓6味中药组成, 经熬制后每毫升含生药1.5 g按8.1 g/kg灌胃。 1.3 指标的检测 1.3.1 血清睾酮含量的检测大鼠经内眦取血, 离心取上清，采用ELISA方法检测大鼠血清睾酮含量。 1.3.2 睾丸