脂肪间充质干细胞治疗外伤性脑损伤.doc

脂肪间充质干细胞治疗外伤性脑损伤 朱俊卿，洪 军，崔建忠，王凯杰(河北医科大学附属唐山工人医院，河北省唐山市 063000) 引用本文：朱俊卿，洪军，崔建忠，王凯杰. 脂肪间充质干细胞治疗外伤性脑损伤[J].中国组织工程研究，2017，21():71-76. DOI: 10.3969/j.issn.2095-4344.2017.01.013 ORCID: 0000-0002-1743-162X(朱俊卿) 文章快速阅读： 文题释义： 脑源性神经营养因子：是1982年Barde等首先在猪脑中发现的一种具有神经营养作用的蛋白质。脑源性神经营养因子及其受体在神经系统广泛表达。一种小分子二聚体蛋白质脑源性神经营养因子结构、分布及信号转导脑源性神经营养因子分子单体是由119个氨基酸残基组成的分泌型成熟多肽，蛋白等电点为9.99，相对分子质量为3.5×103主要由β折叠和无规则卷曲二级结构组成，含有3个二硫键，为一种碱性蛋白质。脑源性神经营养因子分布在中枢神经系统、周围神经系统、内分泌系统、骨和软骨组织等广泛区域内，但主要是在中枢神经系统内表达，其中海马和皮质的含量最高。 胶质细胞源性神经营养因子：于1993年由Lin等从大鼠神经胶质细胞系B49的培养液中首先纯化并命名。目前已在多种神经细胞和神经相关细胞的培养中发现胶质细胞源性神经营养因子表达，并有靶源性神经营养因子的作用。 Adipose mesenchymal stem cells for treatment of traumatic brain injury Zhu Jun-qing, Hong Jun, Cui Jian-zhong, Wang Kai-jie (Tangshan Gongren Hospital of Hebei Medical University, Tangshan 063000, Hebei Province, China) Abstract BACKGROUND: Clinical and animal studies have confirmed that transplanted mesenchymal stem cells can migrate to the area of brain injury, and exert a certain therapeutic effect on traumatic brain injury. OBJECTIVE: To study the therapeutic effect of adipose mesenchymal stem cells on the local damage induced by traumatic brain injury in rats. METHODS: According to the literatures, the brain injury model of Sprague-Dawley rats was made by brain freezing injury. After successful modeling, the rats were randomly divided into three groups: model rats in experimental group were administrated with transplantation of adipose mesenchymal stem cells via the tail vein at 2 days after modeling; model rats in control group given the same amount of normal saline; and normal rats in normal group given no treatment. Morris water maze test was used to evaluate the recovery of neurological function in rats. The isolated and cultured adipose mesenchymal stem cells were observed under an inverted microscope. The distribution of these cells in the injured brain and the levels of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (G