大豆异黄酮分离纯化工艺的研究论文.pdfVIP

C024 大豆异黄酮分离纯化工艺的研究 1 2 2 3 江 英 武占省 李 春 李 新 （1石河子大学食品工程学院； 2石河子大学生物化工实验室；3石河子市卫生防疫站 新疆 石河子 832003） 摘 要 以去除脂肪、蛋白质、可溶性糖、盐等杂质进行了大豆异黄酮的分离纯化工艺研究。去 除脂肪采用石油醚：乙醚＝1：1 的混合溶剂，二次萃取脂肪效果较好；去除蛋白质利用等电点法， 逐步调控 pH=4.3 、4.5 、4.7 使不同种类的蛋白质沉淀，离心后蛋白质去除率可达 90% 以上，而大 豆异黄酮保持率达 93.1% ；利用水解与 PHD600 极性大孔树脂吸附相结合的方法进行脱糖和脱盐； 采用 30%—70%乙醇进行梯度洗脱，发现乙醇浓度 50%和流速为 1.5ml/min 时洗脱效果最好，制 得的大豆异黄酮纯度达 48% ，回收率达 85% 以上。 关键词 大豆异黄酮 分离 纯化 工艺优化 STUDYING ON SEPARATION AND PURIFICATION OF SOYBEAN ISOFLAVONES JING Ying, WU Zhansheng, LI chun and LI Xin （1.Food engineering college of Shihezi University 2.Lab. Of Biochemical Engineering, Shihezi University.3.sanitary epidemic prevention station of Shihezi, Shihezi 832003, Xinjiang） Abstract The process of soybean isoflavones were studied by removing fat, protein, soluble sugars and the other impurity from the ethanol extract solution. Fat of mixture was extracted by solvent of petroleum ether and diethyl ether (1:1) for tow times; protein was removed by combining of isoelectric precipitation and centrifugation and the result showed that removal rate of protein attained over 90% with retain rate of soybean isoflavones 93.1%.;sugars and salt of mixture were removed by hydrolyse and PHD600 pole macroporous resin adsorption; soybean isoflavones were gradient eluted by 30-70% ethanol but 50% and with flow rate of 1.5ml/min best. Eluted solution was collected and made into product . The result showed that purification degree and the yield of soybean isoflavones were over 48% and 85%,respectively. Key words soybean isoflavones Separation Purification Process optimization 引 言 大豆异黄酮是大