单克隆抗体开发高效AlphaLISA免疫检测方法-Human.PDFVIP

Use of the JANUS Automated Workstation to Develop Efficient AlphaLISA Immunoassays for Isotyping Human Monoclonal Antibodies Jessica Suschak, Bagna Bao, Jun Zhang, Jason Hu, Ryan Buff, Philippe Roby, Michael Lässle and Sylvie Kossodo Life Sciences and Technology, PerkinElmer, Inc., Hopkinton, MA Introduction 1 Protocol 7 Performance in Various Cell Culture Media Operator prepares stock solutions of all required reagents (analyte, Immunoassays are commonly used in determining the acceptor beads, biotinylated antibody, donor beads) isotype of monoclonal antibody produced via hybridoma technology. There is an increasing need for efficient and sensitive assays to determine the isotype JANUS® combines 5 µL of analyte with 10 µL of AlphaLISA anti-analyte acceptor beads and 10 µL of biotinylated anti-analyte antibody of these monoclonal antibodies. The most common technology for performing immunoassays is the Incubate 60 minutes at 23°C enzyme-linked immunosorbent assay (ELISA), which is JANUS® adds 25 µL streptavidin Alpha donor beads to each well in a single a time-proven method but requires multiple and time-