水杨酸对梨SOD、PPO 同工酶和NPR1 表达的影响.pdfVIP

园 艺 学 报 2012 ，40 （1）：41 –48 http: // www. ahs. ac. cn Acta Horticulturae Sinica E-mail: yuanyixuebao@126.com 水杨酸对梨 SOD、PPO 同工酶和NPR1 表达的 影响 高丽娟，张玉星* （河北农业大学园艺学院，河北保定 071001 ） 摘 要：以鸭梨（Pyrus bretschneideri ‘Yali ’）为试材，研究外源水杨酸处理后梨叶片中内源水杨酸 （SA ）及超氧化物歧化酶（SOD ）、多酚氧化酶（PPO ）同工酶的变化特点以及病程相关基因非表达子 1 （NPR1 ）对水杨酸的早期应答反应。结果表明：水杨酸处理后梨叶片中内源 SA 含量升高，其中游离态 SA 含量随处理浓度增大而逐渐升高，但均显著低于对照；结合态 SA 随处理增大而逐渐降低，但均显著 高于对照。SOD、PPO 同工酶酶谱分析结果显示，水杨酸处理后酶谱带表达量增强。接种病菌试验表明， 0.200 mmol · L-1 SA 可以诱导梨增强对轮纹病的抗性，且诱导处理过的叶片受到病菌侵染时 SOD、PPO 酶 活性增强。实时荧光定量 PCR 分析表明，0.200 mmol · L-1 SA 诱导后梨叶片中 NPR1 表达量明显升高，但 NPR1 在茎中的表达受到抑制。 关键词：梨；水杨酸；同工酶；NPR1 基因；梨轮纹病菌 中图分类号：S 661.2 文献标志码：A 文章编号：0513-353X （2013 ）01-0041-08 Effects of Salicylic Acid on the Expression of SOD，PPO Isozymes and NPR1 in Pear * GAO Li-juan and ZHANG Yu-xing （College of Horticulture，Agricultural University of Hebei ，Baoding ，Hebei 071001 ，China ） Abstract ：The objective of the study is to investigate the effect of exogenous salicylic acid （SA ）on superoxide dismutase（SOD ）and polyphenol oxidases（PPO ）isozymes changes and endogenous SA content in pear （Pyrus bretschneideri ‘Yali ’）leaves and to examine the response of NPR1 gene expression to SA -1 treatment. The results showed that SA at 0.200 mmol · L increased content of total SA was increased in pear leaves. SA treatment increased the content of free states SA with the increase of SA concentration gradually，and the contents were significantly lower than that of control. The content of binding states SA was reduced with the increase of SA concentration gradually ，but all treatments were significantly higher than that of control. The isozym