慢性肾衰的中医证型与血中内皮素、一氧化氮关系探讨论文.docVIP

　　慢性肾衰的中医证型与血中内皮素、一氧化氮关系探讨论文 .freeles 【Abstract】 Objective To investigate the changes in circulating endothelin(ET)and nitricoxide(NO)of chronic renal failure and TCM syndromes of chronic renal failure.Methods The level of plasma ET easured using radio immuno assay， NO easured using cadmium reduction bar chromatographic analysis and colorimetry.Results pared a ET level uch higher in 150 patients ET level did not shoe of spleen and kidney-Yang groups and the deficiency syndrome of both Yin and Yang (P＞0.05).The rest of groups displayed the significant difference(P 0.01). The serum NO level a ET level (P＜0.01).The plasma ET level of different TCM syndromes decreased according to the folloe of spleen and kidney-Qi， the deficiency syndrome of both -Qi and Yin， the deficiency syndrome of liver and kidney-Yin， the deficiency syndrome of spleen and kidney-Yang， the deficiency syndrome of both Yin and Yang； NO level increased according to the folloe of spleen and kidney-Yang， the deficiency syndrome of both Yin and Yang ， the deficiency syndrome of liver and kidney-Yin， the deficiency syndrome of both Qi and Yin， the deficiency syndrome of spleen and kidney-Qi， the control group.Conclusion The plasma ET and serum NO may have relations ent of chronic renal failure. The plasma ET level can serve as an objective parameter to determine the degree of chronic renal failure. The serum NO level can reflect the degree of Zang-fu organ damage. 【Key l置入含10%EDTA-Na 230μl和40μl抑肽酶的试管中混匀，4℃离心，2000r/min，离心10min，分离血浆置-20℃冰箱保存待测。用放射免疫法测量ET-1含量(解放军总医院东亚免疫研究所药盒)。以西安生产的FJ2008P型γ-放免仪测定。 1.2.2 NO测定 晨起空腹抽静脉血2ml(试管斜放，血液顺管壁缓慢流入，防止溶血)，37℃温育1h，2000r/min，离心5min，分离血清，置-25℃冰箱保存待测，用镉活化还原比色法测定血清NO-3含量(解放军总医院东亚免疫研究所提供药盒)。 1.2.3 统计学分析 采用F检验，q检验，直线相关分析。所有数据用均数±标准差(x±s)表示。 2 结果 2.1 慢性肾衰病人血中ET、NO与正常人的关系 慢性肾衰组血浆ET为(162.96±5.84)ng/L，显著高于正常对照组(50.237±8.53)ng/L(t=1.654，P＜0.01)，但血清NO水平为(162.06±6.56)μmol/L，与正常人组(63.55±11.47)μmol/L差异显著(t=1.65，P＜0.01)。 2.2 慢性肾衰病人血ET、NO与中医证型的关系 见表1、2。 表1 慢性肾炎患者中医证型