脐血基础与临床研究_贾廷珍.pdfVIP

·10 · 2006 2 26 1 　Chin J Radiol M d Prot, F bruary 2006, Vol 26, No.1 ·· 贾廷珍　刘长安　杨光　严智昌　陈旭芳　李惠平　马力文　张淑兰　王保中 【】　　, 。 　 60 3 , Co γ62 mCy , H-TdR、ELISA 、 、MTT 、(MNC)、 + MNC 、CD34 、、、 T 、 NK 、T NK 、 。 　 100～ 150 ml 95.4%, + MNC , 6℃3 d , MNC 91.2%;CD34 , ;T NK , ;, IL-3 ; 。 　, 。 【】　;　 ;　T ;　NK ;　 Basic and clinical researches of human cord blood　JIA Ting-zhen LIU Chang-an YANGGuang, et al.The Third Hospital of Peking University, eijing 100083, China 【Abstract】　Objective　To provid basis for clinical application of cord blood.Methods　Using fr shly isolat d mononucl ar c lls without or with 62 mGy low dos irradiation by 60 Co γ-rays and with 3H-TdR incorporation, r l as assay, ELISA, flow cytom try, MTT and CFU-GM w r p rform d to study th quantity of cord blood, th numb r of MNC, activ MNC p rc ntag , activiti s of T c lls and NK c ll, th xpr ssions of m mbran mol cul s of T lymphocyt s, l v ls of IL-2 TNFα, sIL-2R, cytotoxity of LAK c lls aganst targ t c lls (K562, HL-60), CFU-GM, activity chang s of T c lls and NK c lls aft r irradiation by low dos γ-rays, activity of colony-stimulating factors, r sp ctiv ly.Results　Th quantity of cord blood was rang d 100-150 ml, and th r was no r lation to th MNC numb r.Th cord blood was rich in h matopoi tic st m prog nitor c lls. Immunological activiti s of T and NK c lls was low, but th y w r nhanc d aft r low dos γ-ray irradiation.Th s rum of cord blood was rich in colony-stimulating factors, chi fly IL-3.Th clinical application of cord blood for tr atm nt of l ukop nia would b obviously ff ctiv .Conclusion　Th prosp ct for clinical application of human cord blood is ncouraging. 【Key words】　Human cord blood;　H matopoi tic st m prog nitor c lls;　T lymphocyt s;　NK c lls; 　Low dos irradi