疟原虫—培训课件.pptVIP

参考文献 [1]Site-specific genome editing in Plasmodium falciparum using engineered zinc-finger nucleases， Judith Straimer, Marcus C S Lee, Andrew H Lee, Bryan Zeitler, April E Williams, Jocelynn R Pearl, Lei Zhang, Edward J Rebar, Philip D Gregory, Manuel Llinás, Fyodor D Urnov David A Fidock [2] Branched tricarboxylic acid metabolism in Plasmodium falciparum，Kellen L. Olszewski, Michael W. Mather, Joanne M. Morrisey, Benjamin A. Garcia, Akhil B. Vaidya,Joshua D. Rabinowitz Manuel Llina′s [3]朱晓彤，恶性疟原虫表面抗原SURFINs蛋白家族各成员红内期转录水平的分析 [4]朱晓彤，恶性疟原虫SURFlN4.1重组蛋白在红细胞胞浆内的转运机制的研究 [5]任孝倩，潘卫庆，疟原虫入侵红细胞的相关分子及其机制，国际医学寄生虫病杂志2008年7月第35卷第4期 [6] Moving in and renovating: exportingproteins from Plasmodium into host Erythrocytes，Daniel E. Goldberg and Alan F. Cowman [7]陈军虎，胡薇，恶性疟原虫裂殖子蛋白相互作用的研究进展，国际医学寄生虫病杂志2012年3月第39卷第二期 [8]张亚琼，曹雅明，郑丽，一氧化氮对宿主抗疟原虫感染用机制的研究进展，国际医学寄生虫病杂志2011年9月第38卷第5期 [9]朱晓彤， TLR9介导氯喹的抗疟保护性免疫应答 [10]孙斐，许兵红，Toll样受体与疟原虫侵染关系的研究进展，中国吸血虫病防治杂志2012年第24卷第3期 [11]疟疾疫苗Mosquirix，药学进展，2012年第36卷 Directed genome editing requires the coordinated expression of two ZFNs that heterodimerize to cleave a unique site. Deletion of a key proline residue in the 2A peptide crippled cotranslational release, resulting in a mRFP-2AΔP-GFP fusion protein with a molecular mass slightly greater than the conventional fusion of these two proteins Flow cytometry of the bulk cultures revealed the complete loss of fluorescence in all NF54Δegfp lines . Three independent transfections with a ZFN-deficient control pegfp-hdhfr plasmid failed to yield parasites after 60 d. * PCR and Southern blot analyses nonetheless confirmed replacement of egfp with the mrfp fusion in the majority of parasites, shown in two representative clones (Fig. 2d,e). * The extensive worldwide use of CQ in malaria treatment has led to the selection of multiple mutations in pfcrt17. Genetic engineering of isogenic parasites expressing various pfcrt alleles is required to fully analyze their phenotypic impact on drug respon