响应面法优化黄河鲤鱼鱼鳞酸溶性胶原蛋白提取工艺.pdfVIP

94 20日~ Vol.34, No.20 ※王艺技术 自由融事 响应面法优化黄河鲤鱼鱼鳞酸溶性 胶原蛋白提取王艺 朱文学，邱园园，肖 枫，康帅飞，王玲玲 (河南科技大学食品与生物工程学院，河南洛阳 471023) 摘 要z 以新鲜的黄河鲤鱼鱼鳞为原料，在单因素试验的基础上，采用响应面法Box-Behnken试验设计，对影响 酸溶性胶原蛋白提取效率的3个影响因素即乙酸浓度、液料比和提取时间进行优化，建立并分析各因素与酸溶性 胶原蛋白得率关系的数学模型。结果表明:黄河鲤鱼鱼鳞酸溶性胶原蛋白的最佳提取工艺条件为提取剂乙酸浓度 0.54mol/L、液料比10: 1(mUg)、提取时间42h，在此条件下胶原蛋白的提取率达到15.89%。 关键词:黄河鲤鱼鱼鳞:胶原蛋白:响应面法:提取工艺:优化 Optimization of Extraction Process for Acid-Soluble Collagen 仕om Cyprinus carpio Haematopterus Scale by Response Surface Methodology ZHU Wen-xue , QIU Yuan-yuan , XIAO Fe吨， KANGShuai也i ， WANG Ling-ling (College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023 , China) Abstract: on the basis of one-factor-at-a-time experimen邸， Box-Behnken design (BBD) and response surface methodology (RSM) were appliedωexplore 由e effects of acetic acid concentration, materiallliquid ratio and extraction duration on the extraction efficiency of collagen from ωImmonωrp fish 比a1e. As a result, a mathematica1 model was fit时.币leop也na1 ex位action conditions were found to be 0.54 mol/L acetic acid as the extraction solvent 回thama能riallliquid ratio of 1:10 (g!mL) for an extraction duration of 42 h. Und町these conditions, the extraction efficiency of collagen was 15.89%. Key words: Yellow River common c组p sca1e; collagen; response surface methodology; extraction pl田ess; optimization 中图分类号: TS209 文献标志码:A 文章编号: 1002-6630(2013)20-0094-05 doi:l0.7506/spkxl∞2-6630-201320018 汇，可是却浪费了宝贵的资源[8-12] 。为了提高对水产品 胶原蛋白也称胶原，是由动物细胞合成的一种透明、 资源的利用，同时避免对环境的污染，对水产品加工生 白色、无分支的纤维结构物质。广泛存在于动物的皮肤