响应面试验优化单半乳糖基甘油棕榈酸酯的酶法合成工艺.pdfVIP

※王艺技术 自自酣军 2015, Vo1.36, NO.18 1 响应面试验优化单半乳糖基甘油棕榈酸醋的 酶法合成王艺 齐丹萍，刁明明，陆兆新，吕凤霞，别小妹，张 充，赵海珍* (南京农业大学食品科技学院，江苏南京 210095) 摘 要:以实验室自制的单半乳糖基甘油和游离棕榈酸为原料，在有机溶剂中以固定化脂肪酶Novozyme 435为催 化剂，研究单半乳糖基甘油棕榈酸酷的合成条件。在单因素试验的基础上，以单半乳糖基甘油棕榈酸醋产率为响应 值，确定酶添加量、底物物质的量比和反应时间作为影响合成反应的主要因素，进行响应面优化试验。获得单半 乳糖基甘油棕榈酸酶的最佳合成条件为:丙酣为反应溶剂，脂肪酶Novozyme 435添加量12.93 mg/mL、底物物质 0 的量比(单半乳糖基甘泊-棕榈酸) 1. 00:4.49 、反应温度55 C 、反应时间27.60 h ，此时单半乳糖基甘油棕榈酸醋 产率为90.17% 。 关键词:单半乳糖基甘泊棕榈酸醋;酶法合成:丙酣:脂肪酶Novozyme 435; 优化 Response Surtace Optimization of Lipase-Catalyzed Synthesis of Palrnitoyl-Monogalactosylglycerol in Organic Solvent QI Danping , DIAO Mingming , LU Zhaoxin , LU Fengxia, BIE Xiaomei , ZHANG Chong , ZHAO Haizhen* (College of Food Science and Technology , Nanjing Agricultural University , Na时ing 210095 , China) Abstract: The enzymatic synthesis of palmitoyl-monogalactosylglycerol from monogalactosyl glycerol and free palmitic acid in organic solvent was inv巳stigated in present study. Novozyme 435 , an immobilized lipase , was used as catalyst. The main factors that influence the yield of palmitoyl-monogalactosylglyc巳rol ， including lipase amount, and molar ratio of monogalactosyl glycerol to palmitic acid and reaction time , were selected and optimized by the combined use of single factor experiments and response surface methodology. The optimal synthesis conditions were determined as follows: reaction solvent, acetone; Novozyme 435 amount, 12.93 mg/mL; molar ratio monogalactosylglycero to palmitic acid , 1.00:4 .49; reaction temperature , 55 oC; and reaction time , 27.60 h. Under these conditions , the yield of palmitoyl- monogalactosyIglyc巳r