响应面试验优化碱溶酸沉法提取山药储藏蛋白工艺.pdfVIP

2015. Vo1.36, No.16 7 ※王艺技术 画面融事 响应面试验优化碱溶酸沉法提取 山药储藏蛋白王艺 赵月11 ，高琦3 ，王晓文2 ，贾有峰2 ，张俊伟2 ，艾辛梓2 ，薛友林M (1.辽宁大学生命科学院，辽宁沈阳 11∞36; 2.辽宁大学轻型产业学院，辽宁沈阳 11∞36; 3.辽宁行政学院，辽宁沈阳 110161 ) 摘 要z 以河南焦作白玉山药为原材料，在单因素试验的基础上，采用响应面法中的Box-Behnken试验设计，对影 响山药储藏蛋白提取率的3 个影响因素即抽提pH值、液料比和抽提时间进行优化。结果表明:各因素对山药储藏蛋 白提取率有显著影响，因素影响主次顺序为2 抽提pH值液料比抽提时间，提取山药储藏蛋白的最佳工艺条件 为抽提pH8.5、液料比3 :1 (mUg) 、抽提时间20 min，在此条件下山药储藏蛋白提取率的最大理论值为57.88% ， 实际蛋白提取率为57.74%。所得山药储藏蛋白提取回归模型显著，拟合性好，可用于预测山药储藏蛋白提取率。 关键词:山药储藏蛋白:响应面法:提取工艺;优化 Optimization of Extraction Conditions for Yam Storage Proteins through Alkali-Extraction and Acid- Precipitation by Response Surface Methodology 1 2 2 2 ZHAO Yue .2, GAO Qe, WANG XiaowenZ, JIA Youfeng , ZHANG Junwee , A1 Xinzi , XUE Youlin .* (1. College ofLife Science, Liaoning University , Shenyang 11∞36， China; 2. College of Light Indus町， Liaoning University , Shenyang 11∞36， China; 3. Liaoning Administrative College, Shenyang 110161, China) Abstract:ηle extraction of storage proteins from ‘ Bai归， yam tubers grown in Jiaozuo, Henan province was optimized using combination of one-factor-at-a-time method, Box-Behnken design (BBD) and response surface methodology (RSM). Three factors influencing the extraction yield of y缸n storage proteins , i.e. , pH , liquidJmateria1 ratio and extraction time were investigated. The results showed that a1 1these three factors had a notable effect on 由e ex缸action yield of y缸n storage proteins and their significance could be ranked in a decreasing order of pH liquid