响应面试验优化丹参中多糖的超声波提取工艺及其抗氧化活性.pdfVIP

※王艺技术 2015, Vo1.36, NO.18 7 自曰酣雪 响应面试验优化丹参中多糖的超声波提取 王艺及其抗氧化活性 王燕华，武福华，郭昭涵，彭明星，宋万华，夏 敏，梁子安，张乃群 (南阳师范学院生命科学与技术学院，河南南阳 47306 1) 摘 要:以丹参为原料，利用超声波提取丹参多糖。在单因素试验的基础上，应用Box-Behnken试验设计软件对超 声时间、超声功率、颗粒大小工艺条件进行分析与优化。同时，以1 ， 1- 二苯基-2-三硝基苯阱自由基清除能力、增强 内皮细胞内超氧岐化酶的能力评价超声波法提取丹参多糖的抗氧化活性。结果表明:超声波提取丹参多糖的最优提 取条件为超声功率212 W、超声时间18 min、颗粒大小55 目，此条件下多糖提取率可达4.737毛。抗氧化实验结果表 明，丹参多糖有一定抗氧化活性。超声波浸提法相对单纯热水浸提法可以有效地缩短多糖提取时间，节约能源成本 和时间，同时多糖活性更高。 关键词:丹参:超声提取:多糖:响应面优化;抗氧化 Optimization of Ultrason Íc-Assisted Extraction and Antioxidant Activities of Polysaccharides from the Roots of Salvia miltiorrhiza Bge. WANG Yanhua, WU Fuhua, GUO Zhaohan , PENG Mingxi吨， SONG Wanhua , XIA Min , LIANG Zian , ZHANG Naiqun (College of Life Science, Nanyang Normal University , Nanyang 473061 , China) Abstract: The ultrasonic-assisted extraction of polysaccharides from the roots of Salvia miltiorrhiza with respect to three process parameters including ultrasonication time , ultrasonic power and raw material particle size was optimized using combination of single factor experiments and Box-Behnken design. Meanwhile the antioxidant activity in vitro of the extracted polysaccharides was evaluated by 1,I-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability. The ability to repair endothelial injury induced by high sugar content was analyzed by superoxide dismutase (SOD) activity in endothelial cells. The optimum extraction conditions that provided the maximum yield of polysaccharides of 4.73% were determined as follows: ultrasonic power , 212 W; extraction time , 18 min; and raw material particle size, 55 mesh. Antioxidant assays