原花色素对HepS细胞增殖抑制诱导凋亡的研究.pdfVIP

天然产物研究与开发 Nat Prod Res Dev，2007，19：233-236 文章编号：1001-．6880(2007)Suppl-0233-04 原花色素对HepS细胞增殖抑制诱导凋亡的研究 彭晓光，张太平，彭士明，张鹤云 南京大学生命科学院医药生物技术国家重点实验室，南京210093 摘 要：本文通过体外培养肝癌HepS细胞，以不同浓度原花色素处理12—72 h后，MTI法测定细胞生长抑制作 用，采用DNA片断分析、DNA琼脂糖凝胶电泳、荧光染色以及流式细胞技术等方法来探讨原花色素体外抑制肝 癌HepS细胞及诱导其凋亡的作用。实验结果显示原花色素能抑制HepS细胞的生长，并且呈现出明显的时效 和量效关系，DNA电泳出现典型的凋亡DNA梯形带，在荧光显微镜下，凋亡细胞呈亮绿色，H和AnnexinV．FIFC 双染后，经流式细胞仪检测、分析显示凋亡细胞明显增多。因此原花色素能抑制肝癌HepS细胞株的生长，可能 与诱导其细胞凋亡有关。 关键词：原花色素；HepS；Hoeehest33342；细胞凋亡；DNA琼脂糖凝胶电泳 中图分类号：R739 文献标识码：A Growth Inhibition and Apoptosis Induction of Liver Cancer HepS Cells Line by Proanthocyanidins PENG Xiao-guang，ZHANG Tai-ping，PENG Shi-ming，ZHANG He—yun’ State Key Laboratory ofPharmaceutical Technology，Nanjing University，School of Sciences，Nanjing 210093，China Abstract：Proanthocyanidins are of currevt interest as antieaneer agent．Liver cancer HepS cells line were treated with pmanthoeyanidins with different concentrations for 12-72 h in vitro．Through the method of MIT assay，DNA fragment， DNA agarose gel eleetrophoresis，and flow eytometrie analysis，we can investigate the effect of inhibition and apoptosis induction of pmanthoeyanldins on HepS cells line vitro．MIT assay Was used to measure the effect of the growth inhi- bition and apoptosis of the cells were observed by DNA fragment，DNA agarose gel eleetrophoresis，and flow eytometfie a． nalysis of HepS cells．The results unraveled that proanthocyanidins could inhibit the growth of the cells and present the dependent relationship of time and concentration in the range of time and dose．DNA of the apoptosis cells displayed the DNA ladder tapes by DNA agarose gel electrophoresis．The flow eytometrie analysis of HepS ceHs by Annexin V-FIFC