基质金属蛋白酶对激素治疗特发性肺间质纤维化效果的评价作用.pdfVIP

· 2036· · 论著 · 基质金属蛋白酶对激素治疗特发性肺间质纤维化效果的 评价作用 李晓霞，张捷，李晓艳，智胡，苏振中 【摘要】 目的 探讨基质金属蛋白酶 (MMPs)的表达与激素治疗特发性肺间质纤维化 (IPF)效果的关系。方 法 应用明胶酶谱法检测37例IPF进展期患者 [分为糖皮质激素治疗前组 (IPF，组)、治疗3个月组 (I 组)和治 疗6个月组 (肼 ，组)]和35例健康者 (对照组)血清中以酶原形式表达的明胶酶A(ProMMP一2)和B(ProMMP一 9)及以活性形式表达的明胶酶A(MMP一2)和B(MMP一9)的水平。结果 IPF，组血清ProMMP一9、MMP一9、 ProMMP一2和MMP一2的水平与对照组比较，差异有统计学意义 (P0．05)；IP 和IP 组患者血清ProMMP一9、 MMP一9、ProMMP一2和MMP一2水平与IPF，组比较，差异亦有统计学意义 (P0．05)，但与对照组比较，差异无统 计学意义 (P0．05)。结论 MMPs参与了肺损伤、修复及肺间质纤维化的形成过程，其表达水平与进展期肺纤维化 的程度及激素治疗效果相平行。 【关键词】 基质金属蛋白酶类；糖皮质激素类／治疗应用；肺纤维 药物疗法 【中图分类号】R 563 【文献标识码】A 【文章编号】1007—9572(2007)24—2036—03 Expression of Matrix Metalloproteinases：A Study of Its Evaluation of Glucocorticoid Effect Oil Idiopathic Pnlmonary Fi- brosis LI Xiao—xia，ZHANG Jie，LI Xiao—yan，et a1．Department of Pediatrics，the First Hospital of Hangzhou City， Hangzhou 310006，China 【Abstract】 Objective To investigate the relationship between expression of matrix metalloproteinases(MMPs)and effect of glucocorticoid in treatment of idiopathic pulmonary fibrosis(IPF)．Methods TotMly 37 IPF patients were divided into thine groups：IPF 1 group(without administration of glucocorticoid)，IPF 2 group(administration of glucocorticoid for 3 months)and IPF 3 group(administration of glucocorticoid for 6 months)，and 35 heMthy subjects were enrolled as the contro1． Gelatin Zymography was used to detect the expressions of serum progelationase A(proMMP一2)and B(proMMP一9)，gelation· abe A(MMP一2)and B(MMP一9)．Results The expressions of serum ProMMP一9，MMP一9，ProMMP一2 and MMP一2 in IPF1 group were much higher than those in the control group(P0．05)，and also higher than those in iPF 2 and IPF 3 groups(P0．05)．However，IPF2 group and IPF3 group were not significantly different from the control group in the expres· sions of serum ProMMP一9，MMP一9，ProMMP一2 and MMP一2(P0．05)．Conclusion MMPs may be associat