食管癌放疗前后外周血癌胚抗原mRNA及蛋白检测对比探究.docVIP

食管癌放疗前后外周血癌胚抗原mRNA及蛋白检测对比探究[摘要] 目的：研究食管癌放疗前后外周血癌胚抗原（carcinogenic embryonic antigen，CEA）mRNA与蛋白的表达及其临床意义。方法：收集72例食管鳞状细胞癌（ESCC）患者根治性放疗前后外周血，应用巢式RT-PCR检测CEA mRNA的表达，放免法检测血清CEA蛋白的表达，分析其与临床病理特征及放疗疗效的关系。结果：放疗前CEA mRNA阳性者与CEA蛋白升高者分别占47.2%（34/72）与22.2%（16/72），CEA mRNA的诊断效能显著优于CEA蛋白。并且，放疗前CEA mRNA阳性与淋巴结转移相关（P=0.046），而CEA蛋白升高与食管癌临床病理特征无关。放疗后CEA mRNA与蛋白表达变化均与放疗疗效有关（P=0.034、0.037）。结论：如检测方法得以改进，食管癌外周血CEA mRNA有望成为替代血清CEA蛋白的有效分子标记 [关键词] 食管癌；放疗；癌胚抗原；分子标记 [中图分类号] R735.1 [文献标识码] A [文章编号] 1673-7210（2011）12（a）-015-03 Comparative analysis of CEA mRNA and protein detection in peripheral blood of esophageal cancer patients treated with radiotherapy XUE Jinjun, YUAN Xin, YIN Xudong, WANG Rui, ZHANG Zhengrong, XIA Guangxin Department of Oncology, the First People’s Hospital in Yangzhou City, Jiangsu Province, Yangzhou 225009, China [Abstract] Objective: To study the clinical significance of carcinogenic embryonic antigen (CEA) mRNA and protein expression in peripheral blood of esophageal cancer patients treated with radiotherapy. Methods: CEA mRNA expression was determined by nest RT-PCR and serum CEA protein was determined by immunoassay in pre- and post-radiotherapy blood of 72 esophageal cancer patients, their associations with clinicopathological features and radiotherapy response were further evaluated. Results: The positive expression of CEA mRNA and serum protein in pre-radiotherapy were 47.2% (34/72) and 22.2% (16/72) respectively, the diagnostic value of CEA mRNA was superior to the serum CEA protein. Furthermore, CEA mRNA expression was associated with lymph metastasis in pre-radiotherapy (P=0.046), whereas serum CEA level was not associated with clinicopathological features. Both of the alteration of CEA mRNA and serum CEA protein was associated with radiotherapy response (P=0.034, 0.037). Conclusion: If the detection method can be improved in the future, CEA mRNA expression in peripheral blood maybe a promise biomarker which can replace the serum CEA protein in