HIF-1a 南京医科大学 写成中国了(英文版).pdfVIP

MiR-128 Inhibits Tumor Growth and Angiogenesis by Targeting p70S6K1 1. 2. 2 1 2 2 2 Zhu-mei Shi , Jing Wang , Zhiping Yan , Yong-ping You , Chong-yong Li , Xu Qian , Yu Yin , Peng 1 1 1 3 4 1 1,2,4 Zhao , Ying-ying Wang , Xie-feng Wang , Ming-na Li , Ling-Zhi Liu , Ning Liu , Bing-Hua Jiang * 1 Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China, 2 Department of Pathology, Cancer Center, Nanjing Medical University, Nanjing, China, 3 Department of Pathology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China, 4 Department of Pathology, Anatomy and Cell Biology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America Abstract MicroRNAs are a class of small noncoding RNAs that function as critical gene regulators through targeting mRNAs for translational repression or degradation. In this study, we showed that miR-128 expression levels were decreased in glioma, and identified p70S6K1 as a novel direct target of miR-128. Overexpression of miR-128 suppressed p70S6K1 and its downstream signaling molecules such as HIF-1 and VEGF expression, and attenuated cell proliferation, tumor growth and angiogenesis. Forced expression of p70S6K1 can partly rescue the inhibitory effect of miR-128 in the cells. Taken together, these findings will shed light to the role and mechanism of miR-128 in regulating glioma tumor angiogenesis via miR-128/ p70S6K1 axis, and miR-128 may serve as a potential therapeutic target in gli