衰老分子生物化学中的羰基应激.PDFVIP

7 2 生命科学研究 Vol. 7 No . 2 2003 6 Life Science Research June 2003 李国林, 印大中 ( , 410081) : 羰基应激( 如: 蛋白交联醛氨反应) 是自由基和美拉德反应的共同结果, 也是 老的重要过程. 尽管 生物体本身有羰基降解酶和其它羰基解毒系统, 然而, 即使在健康组织中也还是有大量的有毒羰基化合物存 在, 特别是不饱和醛酮, 如: 马龙二醛4羟基壬烯醛等. 这些不饱和醛酮在生理pH 条件下就能与几乎所有的 重要生物分子( 如: 蛋白质核苷酸等) 自发反应, 导致 一系列与 老相关的变异. 体内的不饱和醛酮还是谷胱 甘肽下降细胞膜损坏酶功能抑制免疫混乱遗传变异细胞复制受阻等病理变化的主要测定指标和重要原 因; 同时也是糖基化终产物老年色素眼球晶体白内障及胶原组织交联等老化现象的前体物质. 因此, 不饱和 醛酮的毒害可能是机体 老的核心过程. : 老; 羰基应激; 糖基化美拉德反应; 自由基 : Q342; R362 : A : 2003) Carbonyl Stress in Molecular Biochemistry of Aging LI Guolin, YIN Dahong ( Laboratory of Aging Biochemistry , Colleg e of Lfi e Sciences, H unan Normal Univ ersity , Chang sha 4 10081, H unan , China) Abstract: Carbonyl stress ( e. g. , crosslinking and aldehydeamino reactions ) of biomolcules, a common consequence of free radicalMaillard reactions, is an important process of biological aging. In spite of detoxif ication of carbonyls by a family of carbonyl dehydrogenases and other biomolcules ( e. g. , glutathione) , even healthy organism contain micromolar amounts of carbonyls, particularly carbonyls with diand multifunctional groups ( DMCs) , e. g. , malondialdehyde, 4hydroxylnonenal, which are all biologically toxic. DMCs react readily at physiological pH with almost all important biomolcules, e. g. , proteins, lipids, carbohydrates, nucleic acids, causing a variety of biological alterations, e. g. , protein crosslinking, which affect most ( if not all) aspects of body organi ation and function.