胃-胰蛋白酶联合酶解阿魏菇蛋白制备抗氧化多肽.docVIP

胃-胰蛋白酶联合酶解阿魏菇蛋白制备抗氧化多肽 王雪铭，许程剑1,*，吴子健2,*，牛博楠1，卢士玲1 （1、石河子大学食品学院，新疆维吾尔自治区 石河子市 832000；2.天津市食品生物技术重点实验室，天津商业大学生物技术与食品科学学院，天津 300134）摘 要：为了建立酶解阿魏菇蛋白制备抗氧化多肽的最佳优化条件，采用胃蛋白酶和胰蛋白酶分步水解的方法。四因素三水平正交试验确定胃蛋白酶的酶解优化组合，以水解度和DPPH自由基清除能力为响应值，最终确定酶解的最优组合为：酶解时间1h，酶添加量8000U/g，底物浓度1%，pH2。在此基础上，采用响应面分析法研究胰蛋白酶酶解时间、酶添加量、pH、底物浓度对工艺的影响，以羟自由基(·OH) 清除率为响应值，确定的酶解最优条件为：酶解时间1.42h，酶添加量7744.93U/g，pH7.96，底物浓度1.35%，此条件下的·OH清除率为74.7%，与理论值75.59%相对误差在1%以内，说明所建立的模型具有较好的拟合性。 关键字：阿魏菇蛋白；抗氧化多肽；响应面分析法；最优条件 Preparation of antioxidant peptides by pepsin-trypsin combined enzymolysis from Pleurotus FerulaeLenzi WANG Xue-ming1，XU Cheng-jian1,*，WU Zi-jian2,*，NU Bo-nan1，LU Shi-ling1 （1. College?of?Food?Science, Shihezi?University, Shihezi, 832000, China; 2. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China）Abstract: In order to establish the optimum conditions of preparation antioxidant peptides from Pleurotus FerulaeLenzi protein, using pepsin and trypsin hydrolysis. The optimum technological conditions of pepsin hydrolysis were obtained by single factor and orthogonal array design methods, the degree of hydrolysis and DPPH radical scavenging capacity for response. The optimum conditions for pepsin hydrolysis method were as follows: hydrolysis time 3h, enzyme dosage 8000U / g, substrate concentration 1%, pH2. On this basis, utilizing response surface for analysis of tryptic hydrolysis time, enzyme dosage, pH, substrate concentration , hydroxyl radical (? OH) as a response to determine the optimal conditions for tryptic hydrolysis as follows: hydrolysis time 1.42h, enzyme dosage 7744.93U/g, pH7.96, substrate concentration 1.35%, in this condition the ? OH clearance was 40.26%, the relative error with the theoretical value 40.86% was less than 1%, indicating that that the model has a good fit of the experiment. Key words: Pleurotus FerulaeLenzi protein; antioxidant peptides; response surface analysis; the optimal conditions 阿