四氧嘧啶腹腔注射致小鼠糖尿病模型因素考察_黄桂红.pdfVIP

年 月第 卷第 期 2012 1 9 1 ·论 著 · 四氧嘧啶腹腔注射致小鼠糖尿病模型因素考察 黄桂红，邓 航，李 江，付 翔，罗昱澜，邹 勇 桂林医学院附属医院药剂科，广西桂林 541001 摘要 目的：探讨四氧嘧啶腹腔注射制备小鼠糖尿病模型的影响因素。 方法：通过腹腔注射四氧嘧啶，研究小鼠性 [ ] 别、体质量、给药剂量、给药次数及不同溶媒等因素对糖尿病小鼠造模成功率、死亡率及血糖的影响。结果：雄性小鼠 较雌性小鼠更适合造糖尿病小鼠模型；给药剂量对造模成功率影响较大，随剂量的增加，小鼠成模率及死亡率均增 高；缓冲液作溶媒成模率较生理盐水成模率高。结论：选择禁食 12 h 的小鼠，以四氧嘧啶185 mg/kg 缓冲液一次性腹 腔注射成模率较高，且糖尿病模型稳定性较好。 关键词 小鼠；四氧嘧啶；糖尿病模型；血糖 [ ] [中图分类号] R332 [文献标识码] A [文章编号] 1673-7210 （2012 ）01 （a ）-015-03 Studies on the factors affecting for establishing diabetic model in mice by in- traperitoneal injection of Alloxan HUANG Guihong, DENG Hang, LI Jiang, FU Xiang, LUO Yulan, ZOU Yong Department of Pharmacy, Affiliated Hospital of Guilin Medical College, Guangxi Zhuang Autonomous Region, Guilin 541001, China [Abstract] Objective: To investigate the factors affecting for establishing diabetic model in mice by intraperitoneal injec - tion of Alloxan. Methods: The effects of the gender, body weight of mice, the dose, times of administration and different solvents were observed in order to evaluate the model successful rate, mortality and fasting blood glucose by intraperitoneal injection of Alloxan in mice. Results: The experiment results showed that the male was more suitable for being induced di abetic model than the female. The dose was the main factor. The model successful rate and the mortality were increasing with the increasing of dose. The solution of Alloxan in buffer was more available than that in physiological saline to induce diabetic mice. Conclusion: A better diabetic mice modle can be gained by intraperitoneal