Folin-Ciocalteu法测定昭通葡萄及其葡萄酒总酚含量.pdfVIP

安徽农业科学，Journal of Anhui Agri. Sci.2014 ,42(33) :11859 -11860 , 11862 责任编辑李菲菲责任校对李岩 Folin-Ciocalteu 法测定昭通葡萄及其葡萄酒总酣含量 袁晓春1 ，李辅碧2 (l 昭通学院，云南昭通 657ω0;2 昭通市食品药品检验所，云南昭通仍7删) 摘要 [目的J Folin-Ciocalteu 法测定苟萄总盼含量，为昭通葡萄生产及其酿制工艺提供科学依据。[方法]采用甲醇作溶剂，超声提取 葡萄总盼，以 Folin-Ciocalteu 试剂和50 glL Na , CO，溶液处理，在 765 nm 处测其吸光度。[结果]以没食子酸为标准，质量浓度在 1 -40 mglL 范围内与吸光度线性关系良好(r =0.999 5) 。乎行测定(n =6) 葡萄总盼含量， RSD 在1. 1 1)奋斗 .6% ，对葡萄和葡萄酒的加标回收 率试验结果分别为 98.8% 、98.3% ，而D 依次为1. 8% 、2.0% 0 [结论]该法操作简便，重复性好，结果准确可靠，可用于测定葡萄及葡萄 酒中总盼含量。 关键词 昭通葡萄;Folin-Ci配alteu 法;测定;总盼 中图分类号 S663.1 文献标识码 A 文章编号 0517 -6611 (2014 )33 -11859-02 Measure of Total Polypbeno1s Content in Zhaoto鸣 Grape 创ld Grape Wine by Folin-Ci侃a1teu Method 1 YUAN Xiao-chun , LI Fu_bi (1. Zhaotong University , Zhaotong ,Yunnan 657αlO; 2. F，∞d and Drug Inspection Office in Zhaotong City , Zhaotong , Yunnan 657αlO) A恼tract [ Objective J In 耐时毗巾0 e曰r tωo 阳pm盯圳d命e scienti凶配fic basi幽剧is ，缸f0盯r pro时duct叫tion 时andb加IreWH吨E in grap严es w副 measured 均by Folin-Cio配ca础lteu method. [Method J Using methyl alcohol as the solvent , total polyphenols of 民tis vinifera w描回国c­ ted by supersonic 酣met血ho叫d ， then added 阳Foωlir0 卧r Basing on galli町c acid , the m幽s concentration had an obvious linear relation (r = O. 阴沟 5) with absorbancy within the scope of 1 - 40 mgl L. The parallel determination (n = 6) of total polyphenol results were that , when RSD w剖 between 1. 1 % to 1. 6% , the adding standard recoveηof Vi- tis vinifera and grapes wine were 98. 8% , 98.3% , RSD 1. 8% , 2.0%. [Conclusion Jη1is method is convenient , repeatable and faithful , wlúch can be used for determination of polyphenol in grapes and grape wine. Key words Zhaotong grape; Folin-Ciocalteu 描say; Determination; Total polyphenol 植物多盼(Plant polyphenol) 又名植物单宁(Vegetable 鲜葡萄，在不同家庭采集以昭通葡萄为原料的家酿葡萄酒。 tannin) ，为植物体内的复杂