HPLC法测定生姜半夏汤单煎与合煎中6-姜酚和6-姜醇含量.pdfVIP

论著生化药物技术 中国生化药物杂志 2015 年第2 期总第35 卷 HPLC 法测定生姜半夏汤单煎与合煎中 6-姜酣和 6-姜醇含量 刘芸1 ，王坷佳2 ，黄错3 ，英正昌l (1.铜仁职业技术学院药学院，贵州铜仁 554300;2. 贵州轻工职业技术学院化工系，贵州贵阳 550025; 3. 贵州理工学院干部科，贵州贵阳 55∞03 ) [摘要] 目的 HPLC 法测定生姜半夏汤单煎液与合煎液中 6-姜盼、6-姜醇的含量变化。方法 采用 Amethyst C旷P 柱 (4.6 mm x250 mm， 5μm) ，以甲醇-水(60:40) 为流动相，检测波长 281 nm ，流速 0.9mVmin，柱温 25 吧。结果 6-姜盼、6-姜醇在合 煎液中含量均明显高于单煎液。结论 生姜半夏汤使用时应采用合煎的方式制备，否则会降低6-姜盼、6-姜醇浸出的含量，影响生姜 半夏汤的药效。 [关键词] 生姜半夏汤;6-姜盼;单煎;合煎;6-姜醇;含量测定 [中图分类号] R286.02 [文献标识码] A [文章编号] 1∞5 -1678(2015)02 -0168 -03 Determination of 6-gingerol and 6-shogaol in ginger pinellia decoction of ditTerent decoction by HPLC 1 2 1 LIU Yun , WANG Ke-jia , HUANG Kae , MO Zheng-chang ( 1. Pharmacy College , Tongren Polytechnic College , Tongren 554300 , China; 2. Department of Chemical Engineering , Guizhou Light Industry Technical College , Guiyang 550025 , China; 3. Department of Cadre , Guizhou Institute of Technology , Guiyang 55α)()3 ， China) [Abstract] Objective To determine the content of 6-gingerol and 6-shogaol in separately decocted and mingly decocted ginger pinellia decoction by HPLC. Methods 咀le samples were separated on Amethyst C旷P (4.6mmx250mm， 5μn) column. The mobile phase was me由anol-water(ω: 40) at the flow rate ofO. 9 mVmin. 四e detection wavelength w脑 281 nm. The column temperature was 25 t:. Results The content of 6-gingerol and 6- shogaol in separately decocted obviously less than that in mingly decocted ginger pinellia decoction. Conclusion Ginger pinellia decoction should be used mingly decocted. Otherwise , it will reduce the contents of 6-ginge