HPLC-电喷雾检测器测定硫酸阿米卡星中卡那霉素和硫酸盐的含量.pdfVIP

.354. 中国抗生素杂志2015年5月第40卷第5期 文章编号: 100 1-8689(2015)05-0354-05 HPLC-电喷雾检测器测定硫酸阿米卡星中卡那霉素和硫酸盐的含量 张凤妹1 王建2. (1 浙江工业大学，杭州 3100 14; 2浙江省食品药品检验研究院，杭州 310∞4) 摘要z 目的 采用HPLC-电喷雾检测器(CAD)新技术测定硫酸阿米卡星中卡那霉素和硫酸盐的含量。 方法 采用华谱C1i ck Xlon(4.6mmX250mm , 5μm)柱，流动相为含0.7%甲酸的250mmollL甲酸镀溶液·乙腊·水(26: 35: 39) ，流速为1 . 0mUmin. 采用 Conona u1tra RS CAD检测器检测，喷雾器温度为25C。结果 卡那霉素、硫酸盐与阿米卡星、中间体、副产物、辅料完全分 离. 卡那霉素、硫酸盐在一定的浓度范围内呈较好的线性关系，卡那霉素测定的回收率为100. 7%(RSD=2.00%， n=9); 硫酸盐测 定的回收率为1 00.2%(RSD=I.04%， n=9). 结论 方法简便、灵敏、重复性好，可用于本品的质量控制. 关键词:硫酸阿米卡星:卡那霉素;硫酸盐; HPLC-电喷雾俭测 中图分类号: R978.1 吃 文献标志码:A The determination of HPLC method for the kanamycin and sulfate in amikacin sulfate using char ged aerosol detector l 2 Zhang Feng-mei and Wang Jian (1 Zhejiang University ofTechno1o町 Hangzhou 31 ∞14; 2 Zhejiang Institute for Food and Drug Contro 1, Hangzhou 310∞4) Abstract Objective A new HPLC- charged aerosol detector technology for the determination of kanamycin and sulfate in amikacin sulfate was developed. Methods The column was Acchrom Click Xlon (4.6mmx 250mm , 印m). The mobile phase was 250mmollL ammonium formate and 0.7% formic acid aqueous solution-acetonitrile- water(26:35:39); the flow rate was 1.0mLlmin. Detector was an Conona ultra RSCAD detector. The sprayer temperature was 25C. Results Good separation of kanamycin and sulfate ftom amikacin and main intermediates could be achieved. The standard curves of kanamycin and sulfate were rectilinear in the certain range. The average recovery of kanamycin is 100.7%(RS D=2.00% , n=9). The average recove