生物破乳剂产生菌的筛选及其方法研究.pdfVIP

带 叫 阳 辙 川 叫 吼 银 JBUM 呻 喃 喃 品 MAY 20 , 2008 , 35(到: 690-695 m m - n y 。 2008 by Institute of Microbiology ,CAS 翩翩 生物破乳剂产生菌的筛选及其方法研究 刘佳黄翔峰*陆丽君闻岳杨殿海周琪 (同济大学环境科学与工程学院罚染控制与资源化研究罔家重点实验盒 上海 200092) 摘 要:针对生物破乳剂产生菌筛选难的问题，采用蓝色法、溶血细胞测试法、表面张力测定法 和排油圈法从6 种不同筒源对生物破乳菌产生商进行了筛选.通过试翰筛选得到了 17 株生物破轧 剂产生菌，典中 24h 内破草L .l!怀高于 70%的破轧菌有 5 株;油田含油污泥、采油废水生物处理污泥 和污水处理f剩余污况是筛选破手L 菌的较好的菌源;蓝色法、雄血圈法存在检测范围的局限性; 表面张力测定法和排油阁法是最为简易和准确的生物表面活性剂产生菌的筛选方法，采用模型导L J民液对生物破乳剂产生菌进行筛选最为直接和准确，但工作轰大、所需时间长，因此在筛选高效 破轧菌时，建议采用农面张力、排油圃法进行初筛，而后通过模型乳收液破草L进行股证。 关键词:生物破草L 剂，表面张力，排油圈法，溶血圈，煤油模型手L状液 Isolation of Strains Producing Bio-demulsifiers and Comparison of Screening Methods LIU Jia HUANG Xiang-Feng* LU Li-Jun WEN Yue YANG Dian-Hai ZHOU Qi (College 01Em例nmental Science Engineel币ng， State Key Laboratory 01Pollution Control αnd Resource Reuse, Tongji Universi纱， Shang阳i2∞092) Abstract: Four screening methods , colorimetric assay, blood-plate hemolysis method, surface tension activ- ity and oi1 spreading technique were introducedωisolate strains producing bio-demulsifiers from 6 different bacteria source s缸nples. 叽le results of various screening methods were evaluated in 也is paper. Seventeen demulsi句ring strains were obtained, whích are qualified in demulsification test of kerosene model emulsions. Among them, 5 strains showed high demulsifying ability, achieving 70% plus demulsifying ratio within 24 hours. Petroleum栅contaminated soil, excess sl