克霉唑乳膏中尼泊金酯含量的测定.pdfVIP

湖南师范大学学报(医学版) 2011.8(4) 71 J Hunan Normal Univ(Med Sci) 克霉瞠乳膏中尼泊金醋含量的测定 曾立华，阳怡锋，刘利民，尹笃林 (湖南师范大学化学化工学院，湖南长沙 41∞81) [摘要]目的:测定克霉哇乳膏中尼泊金酶的含量。方法:高效液相法色谱柱:Alltech C18 柱(4.6 mmx250 mm， 5μm) ，检测波长 :254 nm。用甲醇-水作流动相分析尼泊金酶混合物。结果:用高效液相色谱分析方法测定， 尼泊金甲醋、丙醋的线性范围分别为 4.75-31.67μg/mL和 5.63-37.50μg/mL，平均回收率分别为 100.59%、 99.64%，样品中尼泊金甲苗和丙醋的质量分数分别为 O. 135%(g/g) 、 0.0807%(g/g)，含量测定重复性 RSD 分别为 0.82%、1.82 %。结论:建立了同时测定克霉哇乳膏中对经基苯甲酸、甲爵和丙爵的高效液相色谱分析方法，该方法 操作简单，结果较好，能够广泛应用于尼泊金醋含量的测定。 I 关键词1 尼泊金街;高效液相色谱:测定 [中图分类号) R917 【文献标识码)A [文章编号)1673-016X(2011)04-∞71-03 Quantitative Determination of Parabens in Clotrimazole cream Zeng Lihua, Yang Yifeng, Liu Liming, Yin Dulin (Colloge 01 Chemis町and Chemical Engineering, HuTUJTI Norrnal Univeisitr, CIuuψ0041ω81， China) [Abstract] Objective DetennÏnation of Parahens in Clotrimazole cream in content. Methods: HPLC column: Alltech CI8 column (4.6 mmx250 mm ,5 fJ.m) ,the detecting wavelength was at 254 nm. 白le linear law in the spectra obtained from the high penormance liquid chromatography w描 investigated， using methanol-water 嗣 the mobile phase. The high penormance liquid chromatography method of determining the nipagin and nipasol simultaneously in the Clotrimazole cream w田 estahlished. Results Determination Parahens in the range of 4.75- 3 1.67μg/mL and 5.63-37.50μg/mL， average recovery of 100.59 percent and 99.64 percent respectively, the average content of methyl and propyl parahens in samples are 1.353 mg/g and 0.807 mg/g respectively ,and good reproducibility (espectively RSD 0.829奋，1.82 %) was obtained for the 副say. Conclusion The high penormance liquid chromatograpy method of determing the nipasol and nipasol simultaneously in the Clotrimazole cre