利用香蕉水培系统测定枯萎病病原菌致病力.pdfVIP

植物病理学报 ACTA PHYTOPATHOLOGICA SINICA 44(6); 671-678(2014) doi; 1O.13926/ki.apps.2014.06.014 利用香蕉水培系统测定枯萎病病原菌致病力 杨腊英，郭立佳，毛超，刘磊，王飞燕，谢玉萍，黄俊生* (中国热带农业科学院环境与植物保护研究所，农业部热带作物有害生物综合治理重点实验室，海口 571101 ) 摘要:为建立一种评价香蕉枯萎病病原菌(Fusarium oxysporum f. sp. cubense) 菌株间致病力差异的体系，在改进的香蕉水培 系统基础上，对影响香蕉枯萎病菌致病力的接种菌液浓度、类型及处理方式等因素开展分析，同时与不同菌株盆栽测定结 果进行比较以验证该方法的可靠性。结果表明:在香蕉水堵系统下，将摇培 5d 的菌液稀释 10 倍以上，使抱子初始浓度为 6 J lx10 cfu. mL- ，直接加入该菌液 50 mL即可用于致病力评价，且不同菌株用该测定方法与盆栽测定的致病力结果基本一 致。该方法的建立为香蕉枯萎病菌致病力的评价提供了快速简便的方法，也为下一步解析尖抱镰刀菌致病相关基因功能 和致病机理及抗病品种的选育奠定了基础。 关键词:尖抱镰刀菌古巴专化型;致病力测定;水培系统 Development of a method for evaluating virulence of Fusarium oxysporum f. Sp. cubense in the hydroponic system YANG La-ying , GUO Li才ia ， MAO Chao , LIU Lei , WANG Fei-yan , XIE Yu-ping , HUANG Jun-sheng (Environment and Plant Protection lnstitu饨， Chinese Academy of Tropical Agricultural Sciences , Key Laboratory of Pests Comprehensive Govemance for Tropical Crops , Ministry of Agriculture , Haikou 571101 , China) Abstract: To establish a virulence evaluation method under hydroponic system for Fusarium 0.巧sporum f. sp. cubense based on the improved banana hydroponic culture system , factors affecting pa由ogenicity of F. 。巧ISpO­ rum f.sp. cubense including inoculum concentration , suspension type and treatment method were analyzed , and the results were also compared with those by inoculation of po伽d plants to verify the acc町acy of the hydroponic test. It was indicated that: In banana hydroponic culture system , 50 mL of spore suspension shaking-cultured for 6 5 days with a diluted concentration at 1 x 10 cfu • mL-1 could be used for virulence evaluation , and the results were basically the s组ne as those obtained by potted seedlings measurement.古J.Ïs study provides a quick and e副y way for vi