siRNA转染浓度和时间对HeLa细胞ANXA5表达的抑制作用_王小杰.pdfVIP

INHIBITING EFFECTS OF SIRNA TRANSFECTION CONCENTRATION AND TIME ON ANXA5 EXPRESSION IN HELA CELLS WANG Xiao-jie, WANG Yong-wei, QU Yin-e, et al (Basic Medical Sciences of Hebei United University, Hebei Tangshan 063009, China) ABSTRACTObjective: To optimize the conditions of siRNA targeting ANXA5 transfected into HeLa cells, so to provide bases for further studying effects of ANXA5 low expression on biological functions of cervical cancer cells. Methods:siRNA targeting ANXA5 was transfected into HeLa cells, with different ratios of ANXA5 siRNA and liposomes, and different transfection time. Western Blot was used to detect the ANXA5 protein expression in HeLa cells. Results:The ANXA5 protein expression in HeLa cells was the lowest (the inhibitory effects was the strongest) when the ratio of siRNA and liposomes was 0.4:1 and transfection time was 72h. Conclusions:The ratio of siRNA and liposomes and transfection time can infl uence the expression of ANXA5 in HeLa cells, and has the best ratio and transfection time. KEY WORDSsiRNA; HeLa cells; ANXA5; Inhibiting effects