NGF在人胚胎脊髓中的表达及变化.pdfVIP

2007, ( 4): 1~ 5 CN 53- 1049/R Journal ofKunm ing M dica l Coll g NGF 1) 1) 1) 1) 2) 1) , , , , , ( 1) 昆明医学院神经科学研究所, 云南 昆明 650031; 2) 昆明医学院附属昆华医院, 云南 昆明 650032) [ ] 目的 ( NGF) . 方法 ( 6 8103456, RT- PCR NGF mRNA . 结果 NGF- IR ; RT- PCR NGF mRNA, 6, 8, 10~ 6 . 结论 NGF, NGF. [ ] ; ; NGF; ; RT- PCR; [ ] R651 [ ] A [ ] 1003- 4706 ( 2007) 04- 0001- 05 Th Expr ssions and Chang s of NGF in th F talHuman Spinal Cord 1) 1) 1) 1) 2) 1) LI Li- yan , LIU Jia , YANG Jin- w ei , GAO Yan , GUO Jian- hu i , WANG T ing- hua ( 1) Institute of N eurosc ience, K unm ing M ed ical Colleg e, K unm ing 650031; 2) Aff iliated K unh ua H osp ital of K unm ing M ed ical C olleg e, K unm ing 650032, Ch ina ) [Abstract] O bjec tive To investigate the expressions and changes ofNGF and in pars thoracicamedullae spinalis of the fetal human spinal cord. M e thods Sp inal cordw as taken out from elective abortion foetus at d if- ferent period of 6w eek, 8 week, 10w eek, 3month, 4 month, 5 month and 6month respectively, then mi muno- h istochem istry and RT- PCR w ere used to detect the change law of NGF expression. Resu lts NGF- IR cou ld be seen respectively in gray matter in pars thoracicamedu llae spinalis of the fetal human sp inal cord from 6w eek to 6 month by mi munoh