啮齿类动物手术麻醉.pdfVIP

嚙齒類動物麻醉手術照護 戴元基 教授 林口長庚醫 院實驗麻醉科 Yuan-Ji Day, MD, PhD Chang Gung Memorial Hospital Council, AAALAC International General anesthetic plan • Preparation for anesthesia • Pre-anesthetic drugs • Intraoperative and recovery support • Sequential surgeries on a number of rodents in a single session Special consideration of anesthesia in rodent • Animal consideration – Strain, volume limit, gender, genotype, phenotype, age, light cycle, health status etc • Procedure consideration – Survival or non-survival, scientific goal,physical limitation (MRI) etc • Practical consideration – Equipment, training and expense Useful information Animal characteristics • Characteristics of the individual animal that need to be considered when selecting anesthetics – Species and strain – Age, size, and sex – Physical condition – Temperament – Health (preexisting diseases) – Previous administration of other drugs Genotype influence the anesthetics effects METHODS OF DELIVERY • Parenteral route • Inhalation route • intraperitoneal (IP) • open drop delivery • intramuscular (IM) • precision vaporizer • intravenous (IV) • subcutaneous (SC) Disadvantages of Injectables • Once a given dose is administered, the anesthetic cannot be changed. • The duration of anesthetic effect is individual-dependent. • There is individual variability for a given dose. • Some injectable agents are irritating and measures need to be taken to minimize this effect Inhalant anesthetics- advantages • Increased control over depth as well as duration of anesthesia. • Increased safety and survivability. • Minimal metabolism, biotransformation and excretion compared with injectables,