贮存血小板形态变化与凋亡因子磷脂酰丝氨酸表达的研究.pdfVIP

· 476 · 中国输血杂志2007年 12月第2O卷第6期 Chin J Blood Transfusion．December，2007．Vo1．20，No．6 · 论 著 · 贮存血小板形态变化与 凋亡因子磷脂酰丝氨酸表达的研究 熊文 竺展坤 邬旭群 张印则 周丹 邵超鹏(深圳市血液中心 输血医学研究所，广东深圳518035) 摘要：目的 探讨储存期间机采血小板形态变化、凋亡因子磷脂酰丝氨酸(phosphatidyl serine．PS)表达以及两 者之间的关系，为确立血小板体外保存时限提供实验依据。方法 应用May-Grunwald-Giemsa染色方法观察机采 血小板储存O一8d时的形态变化，同时应用流式细胞术检测血小板的膜 PS表达率。结果 储存至第4天，血小板 形态出现损伤，表现为形态学计分与新鲜血小板相比显著下降(t一2．341，PO．05)；随着储存时间延长，血小板形 态学计分持续下降，至第7天，形态学计分下降31 。血小板凋亡因子PS表达．储存1d组与新鲜血小板Od组相比 有明显升高(t一3．088，P0．05)；储存1—3d．PS表达无明显变化，储存至第4天，PS表达显著增加(t一2．1612。 P0．05)；储存5—8d，PS表达持续增加，各组间有统计学差异(P均O．05)。血小板形态学变化分值与膜 PS的 表达随储存时间延长呈负相关性(r一一0．9923，P0．01)。结论 储存血小板形态学变化分值与膜PS表达率高 度负相关。 关键词：血小板；形态学；磷脂酰丝氨酸；储存；流式细胞术 中图分类号：R457．1 R331．1 43 文献标识码：A 文章编号：1004-549X(2007)06-0476-04 Morphology changes and the expression of phosphatidylserine in stored platelets XjONG Wen，ZHU Zhankun，WU Xuqun，et a1．Transfusion Medicine Institute，Shenzhen Blood Center，Shenzhen，Guangdong 5 18035，China Abstract：Objective To study the morphology changes and the expression of phosphatidyl serine(PS)in aphere— sis platelets(Pits)during storage。also to analyze their relationship，in order to provide laboratorial evidence for the in vitro storage period of pits．Methods The morphological changes of pits during 8-day storage were detected by May-Grunwald-Giemsa dyeing method．and the expression of PS on platelet membrane was tested by flow cytometry (FCM)．Results The morphological lesions appeared on the fourth day of storage．because the modified morphologi— cal score(MMS)of 4-day group was significantly lower than that of fresh pits(t一 2．341，P 0．05)．The MMS kept on decreasing with extended storage。and on the seventh day of storage。the MMS declined to 3 1％．However， the PS expression rate of 1-day storage group was obviously higher than that of 0-day group(t一 3．088，P 0．05)．