降低血清IGF-1水平抑制乳腺肿瘤细胞增殖的实验研究.pdfVIP

中国医师杂志 2007年12月 第9卷第12期 l607 降低血清IGF一1水平抑制乳腺肿瘤细胞增殖的实验研究 唐泓波，任玉萍，张珊，陈国庆，吴毅平一 (华中科技大学同济医学院附属同济医院普外科，湖北 武汉 430030) 【摘要】 目的 在肝特异性胰岛素样生长因子1(IGF-1)基因缺失小鼠及对照鼠体内建立原发性乳腺癌，探讨血清中IGF．1水 平与乳腺肿瘤细胞增殖的关系。方法 使用7，12-二甲基苯蒽(DMBA)在实验鼠体内诱导原发性乳腺癌，人参皂甙RS3进行干预 治疗。比较各组肿瘤发生情况，流式细胞术检测细胞周期及凋亡比例。结果 未管饲RS3的对照鼠乳腺癌发病率为66．67％，显著 高于其他各组(P0．05)；而管饲RS3的肝特异性IGF-1基因缺失(LID)鼠乳腺癌发病率为12．00％，显著低于其他各组(P 0．05)。未管饲RS3的对照鼠凋亡比例为(2．47±0．69)％，低于其他各组(P0．05)；而管饲Rg3的LID鼠凋亡比例为(14．00± 1．74)％，高于其他各组(P0．05)。结论 降低血清IGF-1水平可促进细胞凋亡，对乳腺肿瘤的发生具有抑制作用，人参皂甙Rg3 对这一效应具有协同作用。 [关键词] 胰岛素样生长因子I；乳腺肿瘤；细胞增殖 The experimental study of low serunl IGF·1 level inhibit mammary tumor cell proliferation TANG Hong-bo，REN Yu-ping，ZHANG Shan，CHEN Guo-qing， U Yi-ping．Department of Surgery，Tongii Hospital，Tongii Medical Col- lege，Huzhong University of Science and Technology，Wuhan 430030，China 【Abstract】 Objective A stable primary mammary tumor model in liver-specific insulin-like growth factor I(IGF-1)deficient (LID)mice and control mice was established．The potential relationship between circulating IGF-1 level and proliferation of mammary tumor was explored．Methods Induction of mammary tumors was achieved by using 7，12-dimethybenz(a)anthracene(DMBA)in experimental mice． Ginsenoside RS3 was used in treatment．Th e incidence of mam mary tumor in every group was compared，and the cell cycle and apop- tosis percentage were determined by flow cytometry．Results Th e incidence of tumor in untreated control mice was 66．67％ ，which was sig- nificanfly higher than that in any other group(P0．05)．The incidence oftumor in Rs3-treated LID mice was 12．0o％，which was signifi- cantly lower than that in any other group(P0．05)．The apoptosis percentage in untreated control mice was(2．74±0．69)％，which was significantly lower than that in other groups(P0．05)．The apoptosis percentage in RS3-treate