pGL4.26哺乳动物表达载体说明.pdfVIP

pGL4.26 编号 载体名称 北京华越洋生物VECT6094 pGL4.26 pGL4.26 载体基本信息 载体名称: pGL4.26 、pGL4.26[luc2/minP/Hygro] 质粒类型: 哺乳细胞表达；信号通路报告载体 高拷贝/低拷贝: -- 启动子: 弱启动子 克隆方法: 多克隆位点，限制性内切酶 载体大小: 5882 bp 5 测序引物及序列: -- 3 测序引物及序列: -- 载体标签: -- 载体抗性: 氨苄青霉素Ampicillin 筛选标记: 潮霉素Hygromycin pGL4.26 载体含有荧光素酶报告基因 luc2 和弱启动子，用于研究细胞内信 备注: 号通路中转录应答元件的活性。 稳定性: -- 组成型: 组成型 病毒/非病毒: 非病毒 pGL4.26 载体质粒图谱和多克隆位点信息 pGL4.26 载体简介 The pGL4.26[luc2/minP/Hygro] Vector(a–e) encodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The vector contains a multiple cloning region for insertion of a response element of interest upstream of a minimal promoter and the luc2 gene. luc2 is a synthetically-derived luciferase sequence with humanized codon optimization. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a mammalian selectable marker for hygromycin resistance. pGL4.26 载体序列 ORIGIN 1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC TGGCCTCGGC 61 GGCCAAGCTT AGACACTAGA GGGTATATAA TGGAAGCTCG ACTTCCAGCT TGGCAATCCG 121 GTACTGTTGG TAAAGCCACC ATGGAAGATG CCAAAAACAT TAAGAAGGGC CCAGCGCCAT 181 TCTACCCACT CGAAGACGGG ACCGCCGGCG AGCAGCTGCA CAAAGCCATG AAGCGCTACG 241 CCCTGGTGCC CGGCACCATC GCCTTTACCG ACGCACATAT CGAGGTGGAC ATTACCTACG 301 CCGAGTACTT CGAGATGAGC GTTCGGCTGG CAGAAGCTAT GAAGCGCTAT GGGCTGAATA 361 CAAACCATCG GATCGTGGTG TGCAGCGAGA ATAGCTTGCA GTTCTTCATG CCCGTGTTGG 421 GTGCCCTGTT CATCGGTGTG GCTGTGGCCC CAGCTAACGA CATCTACAAC GAGCGCGAGC 481 TGCTGAACAG CATGGGCATC AGCCAGCCCA CCGTCG