pGL4.30哺乳动物表达载体说明.pdfVIP

pGL4.30 编号 载体名称 北京华越洋生物VECT6046 pGL4.30 pGL4.30载体基本信息 载体名称: pGL4.30 、pGL4.30 [luc2P/NFAT-RE/Hygro] 质粒类型: 哺乳细胞表达；信号通路报告载体 高拷贝/低拷贝: -- 启动子: -- 克隆方法: 多克隆位点，限制性内切酶 载体大小: 6097 bp 5 测序引物及序列: -- 3 测序引物及序列: -- 载体标签: -- 载体抗性: 氨苄青霉素Ampicillin 筛选标记: 潮霉素Hygromycin 备注: pGL4.30载体含有荧光素酶报告基因luc2P和NFAT 应答元件。 稳定性: -- 组成型: 组成型 病毒/ 非病毒: 非病毒 pGL4.30载体质粒图谱和多克隆位点信息 pGL4.30 载体简介 The pGL4.30[luc2P/NFAT-RE/Hygro] Vector(a–f) contains an NFAT response element (NFAT-RE) that drives the transcription of the luciferase reporter gene luc2P (Photinus pyralis). luc2P is a synthetically-derived luciferase sequence with humanized codon optimization that is designed for high expression and reduced anomalous transcription. The luc2P gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly than the protein encoded by the luc2 gene upon induction. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a mammalian selectable marker for hygromycin resistance. pGL4.30 载体序列 ORIGIN 1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCGGAGGAAA AACTGTTTCA TACAGAAGGC 61 GTGGAGGAAA AACTGTTTCA TACAGAAGGC GTGGAGGAAA AACTGTTTCA TACAGAAGGC 121 GTAGATCTAG ACTCTAGAGG GTATATAATG GAAGCTCGAA TTCCAGCTTG GCATTCCGGT 181 ACTGTTGGTA AAAAGCTTGG CAATCCGGTA CTGTTGGTAA AGCCACCATG GAAGATGCCA 241 AAAACATTAA GAAGGGCCCA GCGCCATTCT ACCCACTCGA AGACGGGACC GCCGGCGAGC 301 AGCTGCACAA AGCCATGAAG CGCTACGCCC TGGTGCCCGG CACCATCGCC TTTACCGACG