对氧磷酶-1与2型糖尿病的关系研究.pdfVIP

第l3卷4期 天 津 医科 大 学 学 报 V01．13．No．4 514 2007年l2月 JOURNAL OF TIANJIN MED1CAL UNIVERSITY Del2．20o7 对氧磷酶一1与2型糖尿病的关系研究 李京艳，唐 洁，王 敬，周毓玲，冀秋娣 (天津医科大学总医院代谢病科，天津300052) [摘要]目的：探讨对氧磷酶一l(PON一1)与2型糖尿病(T2DM)的关系。方法：测定63例T2DM患者和33例健康人血 清PON-1活性，并应用聚合酶链式反应一限制性片段长度多态 I~(PCR—RFLP)技术研究其PON—l第191位密码子 基因变异。结果：(1)T2DM组血清PON—l活性低于对照组，差别具有统计学意义(拄2．75，P0．05)。(2)T2DM组与对照 组PON—l第191位密码子基因型分布无显著性差异(x =3．03，PO．05)。结论：T2DM组血清PON—l活性显著低于对 照组，这可能是T2DM患者血管疾病高发的原 之一 [关键词]2型糖尿病；对氧磷酶一l；血清对氧磷酶一l活性；基因多态性 [中图分类号]R587．1 [文献标识码]A [文章编号]1006—8147(2007)04—0514—03 Study on the relationship between paraoxonase-1 and type 2 diabetes melfitus LI Jing—yan，TANG Jie，WANG Jing，ZHOU Yu-ling，JI Qiu-di (Department of Metabolic Diseases，General Hospital，Tianjin Medical University，Tianjin 300052，China) ABSTRACT Objective：To reveal the relationship between serum paraoxonase一1 (PON一1)activity， PON一1 Q／R polymorphism and type 2 diabetes mellitus in Chinese Han ethnic group．Methods：The sub— jects of the study consisted of two groups：(1)type 2 diabetes mellitus group；(2)normal-control group． Serum PON——1 activity was determined using paraoxon as substrate by measuring the increase rate in the absorbance at 405 nm due to formation of 4-nitrophenol，and PON一1 Q／R polymorphism was evaluated by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP)．Results：(1)There was no significant difference in the distribution of the PON-1 Q／R polymorphism between T2DM group and normal—control group =3．03，P0．05)．(2)Serum PON-1 activity in T2DM group and normal—control group was(44．65+19．96)u／ml and(74．85+27．72)u／ml，respectively．Serum PON-1 activity in the former group was significantly low c