瘦素与胎儿生长发育关系的探讨.pdfVIP

山西医药杂志2008年1月第37卷第1期 Shmlxi Medj．january 2008，Vo1．37，No．1 · 23 · 瘦素与胎儿生长发育关系的探讨 山西医科大学第一医院(030001) 何玉洁 陈云霞 成要平 【摘 要】 目的 探讨瘦素与胎儿生长发育的关系。方法 采用放射免疫法检测90例足月剖宫产孕妇母 血及脐血中瘦素水平，根据新生儿出生体重分为小于胎龄儿(S( l7例)、适于胎龄儿(AGA 47例)和大于胎龄儿 (LGA 26例)3组；巢式反转录一聚合酶链反应技术测定2l例胎盘组织中瘦素mRNA的相对表达量(SGA组、 AGA组、LGA组各7例)。采用新生儿出生体重和Ponderal指数估测胎儿生长发育状态。结果 ①母血瘦素水 平与胎儿出生体重、Ponderal指数和胎盘重量无相关关系，与母体体重指数(BMI)呈显著正相关。②脐血瘦素水 平与胎儿出生体重、Ponderal指数、胎盘瘦素mRNA表达量和胎盘重量呈显著正相关，3组间比较，差异有统计学 意义(P0．01)，其中SGA组显著低予AGA组、LGA组显著高于AGA组。③胎盘组织中瘦素mRNA的相对表 达量与胎儿出生体重、Ponderal指数和胎盘重量呈显著正相关(P0．01)，3组间比较，差异有统计学意义(P 0．01)，其中SGA组显著低于AGA组、LGA组显著高于AGA组。结论 母血瘦素水平不能预测胎儿体重，脐血 瘦素水平和胎盘瘦素mRNA与胎儿生长发育密切相关。 【关键词】瘦素；胎儿； 出生体重；胎盘瘦素mRNA Investigatethe relationships betweenleptin andfetal growth HEYu-jie，CHENYun—xia，CHENGYao-ping．First Affiliated Hospital ofShanxi Medical University， iyuan 030001，China [Abstract]Objective To investigate the relationship between leptin and fetal growth and development．Meth- ods The levels of matemal blood leptin and cord blood leptin in 90 healthy full—term deliveries were examined by radio immuncxassay(RIA)．A1l subjects were categorized aS SGA(17 cases)，AGA(47 cases)and LGA(26 CSSes)according tO birth wei ght．The relative expression of leptin—mRNA in 21 placenta，include SGA(7 cases)，AGA(7 cases)and LGA(7 eases)，determined by．semi—quantitative nested RT-PCR．Results ①Maternal leptin was markedly positively correlated with maternal BMI，but not with birth weight，Ponderal index and placental weight．② Ird leptin was markedly 15ositively corrdated with birth weight，Ponderal index and placental weight，the expression of leptin—rn~NA in placenta and placental weight．Among three groups，there WS significant difference(P0．01)，．Cord leptin in LGA group and in SGA group WS，respect