モノリス型シリカカラムの调制と性能评似 preparation and evaluation .pdfVIP

CHROMATOGRAPHY, Vol.32 No.2 (2011) Technical Review Preparation and Evaluation of Monolithic Silica Columns for HPLC (1) (1) (1)(2) (2) Shota Miyazaki *, Shigenori Ota , Kei Morisato , Kazuki Nakanishi , Masayoshi Ohira(1) , Nobuo Tanaka(1)(3) (1) GL Sciences Inc., 237−2 Sayamagahara, Iruma, Saitama, 358−0032, Japan (2) Kyoto University, Sakyo−ku, Kyoto 606−8502, Japan (3) Kyoto Institute of Technology, Sakyo−ku, Kyoto 606−8585, Japan abstract Monolithic silica columns have been developed to provide the performance beyond the limit of conventional particulate columns along with the columns packed with sub−2 µm particles or core−shell particles. This review describes (1) the preparation method of monolithic silica gel, (2) the surface modification method, (3) the method of column fabrication, and (4) the evaluation method of column performance. Keywords : monolithic silica column, silica preparation, surface modification, column fabrication, column evaluation sub µm