周传农修改 - 中国实验动物学报.docVIP

周传农修改： 英文摘要 — 已修改 正文中的英文书写 — 已顺 图与表的英文 — 已顺。表1需请作者补充英文。 文献的英文书写 — 已顺。有些文献需请作者补充。 应激所致肠功能紊乱大鼠模型的建立及内源性标志物的评价 周晨，胡浪，李天雪，张楚筠，杨星昊 （南京师范大学生命科学学院，新药研究中心） 【摘要】 目的：探讨应激所致肠功能紊乱大鼠模型建立和评价的方法。方法： SD大鼠随机分为肠功能紊乱模型组和正常组，模型组采用慢性束缚+过度疲劳+饮食失调法造模，3周后取血，用酶联免疫法（ELISA）试剂盒在血清测GAS、IL-2，在血浆中测ACTH、MTL、SS。结果：模型组大鼠3周后与正常组相比，外观行为发生明显变化，体重和肝脾重量显著降低，生物标志物水平降低。结论：通过慢性束缚+过度疲劳+饮食失调法可以建立稳定的应激肠功能紊乱大鼠模型, GAS、IL-2、ACTH、MTL、SS等内源性物质可作为模型的评价指标。 【关键词】 肠功能紊乱，动物模型，生物指标 Establishment of a rat model of stress-induced intestinal dysfunction and evaluation of the endogenous biomarkers Zhou Chen, Hu Lang, Li Tian-xue, Zhang Chu-yun, Yang Xing-hao (New Drug Research Center, Nanjing Normal University School of Life Science, Nanjing 210046, China) 【Abstract】 Objective xplore the method of establishment of a rat model of stress-induced intestinal dysfunction and the method of its evaluation. Methods Sprague-Dawley rats were randomly assigned to two groups: the normal group and the model group. The model group was developed by combination of chronic constraint, excess fatigue and eating disorders for three weeks. At the end of the third week, the serum GAS, IL-2, and plasma MTL, SS and ACTH were measured using an ELISA kit. Results Compared with the normal group, the model group had many distinct changes of appearance and behavior, weight loss and decrease of liver and spleen weight, and apparent changes of many biomarkers. Conclusions Stable rat models of stress-induced intestinal dysfunction can be successfully established by combination of chronic constraint, excess fatigue and eating disorders in SD rats, and the biomarkers such as GAS, IL-2, ACTH, MTL, SS can be used to evaluate this model. 【Key words】ntestinal dysfunction; Animal model; Biomarkers; Rat [基金项目] 国家基础科学人才培养基金 (J1103507，J1210025) [作者简介]周晨（1988年- ），男，硕士研究生。E-mail: zhou001212@126.com。E-mail: yangxinh@126.com 肠易激综合征( irritable bowel syndrome, IBS)是( 腹泻、便秘或腹泻与便秘交替) 和( 或) 大便性状的改变等为主要临床表现, 经检查无特异的生物化学或组织学异常[1] 。大量的研究表明，IBS病因涉及精神因素、神经内分泌等因素[2-5]，其发病的机制主