Elicitin诱导烟草微敏反应和防卫基因的表达.pdfVIP

植物病理学报 ACTA PHYTOPATHOLOGICA SINICA 37(6)：610-615(2007) E l iciti n诱导烟草微敏反应和防卫基因的表达 梁元存 ，刘爱新 ，王金生 ，张天宇 ，董汉松 ( 山东农业大学植物病理学系，泰安271018；。南京农业大学农业部病虫检测与治理重点开放实验室，南京210095) 摘要：研究了一种ot型elicitin—parasiticein对烟草微敏反应(microscopic hypersensitive response，micro．HR)和防卫反应分子 表征基因表达的诱导作用。用150 nmoVL的parasiticein喷洒烟草叶片12 h后，经曲利本蓝(trypan blue)染色，光镜下可观 察到有细胞坏死，说明parasiticein引起了micro—HR，而水和低浓度的parasiticein不能引起micro—HR。用parasiticein注射叶 片可引起肉眼可见的HR，注射用parasiticein浓度远比喷洒引起micro．HR的浓度低。Parasiticein还可诱导对TMV的抗 性，浓度在30 nmoVL时比l50 nmoVL的效果好。用parasiticein注射烟草30 min，HR表征基因hsr203J和hinl开始表达， 在9 h内逐渐降低，l2—16 h肉眼可观察到HR。Parasiticein喷洒烟草后，病程相关蛋白(pathogenesis．related，PR)基因PR— J易在诱导的第l d到第5 d都有一定量的表达。可见，parasiticein能同步诱导过敏反应和抗病性及其分子表征基因的表达。 关键词：elicitin；parasiticein；微敏反应(micro—HR)；防卫反应 Microscopic hypersensitiVe response and expression of defense response induced by the pr0teinace0us elicitor elicitin—parasiticein LIANG Yuan—cun ，LIU Ai—xin ，WANG Jin—sheng ，ZHANG Tian—yu ，DONG Han—song ( Department of Plant Pathology，Shandong Agricultural Universi— ty，Taian 271018，China； Key Laboratory of Monitoring and Management of Plant Diseases and Insects，Ministry of Agricul— ture，Nanjing Agricultural University，Nanjing 210095，China) Abstract：Elicitins produced by species of plant pathogenic oomycetes constitute a unique group of small pro— teins that induce hypersensitive response(HR)and pathogen defense in plants．Modes and molecular bases of the responses have not been well understood．Here we studied a particular elicitin，parasiticein from P toph— thora parasitica var．nicotianae，and focused on modes of HR and pathogen defense that the protein causes． To illustrate whether parasiticein induces microscopic hypersensitive response(micro—HR)，tobacco leaves were sprayed with parasiticein，stmned with trypan blue，and observed by light mi croscopy．Micro—HR was observed on leaves in 12 h after treatment with a moderate dose of parasiticein．Inv