hLRH-1调控结肠腺癌细胞SW480增殖的分子机制.pdfVIP

·904· 肿瘤防治研究2007年第34卷第12期 hLRH一1调控结肠腺癌细胞SW480增殖的 分子机制 郑英梦 ，王水良，兰风华 Molecular Mechanism of hLRH-1 in Regulating Proliferation of Colon Adenocarcinoma Cells SW480 ZHENG Ying-meng ，WANG Shui—liang，LAN Feng—hua Center r Molecular Medicine，Institute o f Laboratory Medicine，Fuzhou General Hospital，Fuzhou 350025，China( Present：Department of Laboratory Medicine，First Hospital of Quxznzhou。 Ql zhOU 362000，C ina) Corresponding Author：WANG Shui-liang，E-mail：shuiliangwang@yahoo．corn．cn Abstract：Objective To explore the possible biological function of human nuclear receptor hLRH一1 in tu— morigenesis and progress of colon cancer．Methods Plasmids pcDNA3一hLRH一1 were introduced into S、Ⅳ480 cells via lipofectamine．The expression of mRNA and protein of exogenous hLRH一1 was detected by RT-PCR and Western blotting，respectively．MTT assay was carried out to survey the proliferation of SW480 cells with overexDression of hLRH一1．Meanwhile，the expression of proliferation-related genes cyclin E1 and cyclin D1，and apoptosis—related genes PTEN and Rbl，were analyzed by real-time RT- f℃R Results The proliferation of SW480 cells were promoted under the condition of overexDression of hLRH-1．The expression of cyclin E1 was up-regulated significantly。while that of Pten and Rbl was down-regulated in SW480 cells with overexpressed hLRH一1．Conclusion The expression of exogenous hLRH一1 in SW480 cells induced the proliferation resulting form up-regulation of cyclin E1。as well as participated in the regulation of apoptosis via influencing the expression of Pten and Rb1． Key words：Nuclear receptor hLRH一1；Colon cancer；Expression of exogenous gene；Proliferation 摘 要：目的 初步探讨人核受体hLRH一1在结肠癌发生发展中可能的生物学功能。方法 真核表达 质粒pcDNA3一hLRH-1经脂质体介导转入SW48()细胞中，RT_PCR和Western blot法分别检测外源基 因mRNA及蛋白水平的表达；M1vr法分析hLRH一1过表达对SW480细胞生长增殖的影响，同时行实 时定量RT-PCR法分析细胞生长增殖相关基因Cyclin E1和Cyclin D1、以及凋亡调控相关基因Pten和 Rbl的表达。结果 hLRH一1过表达的SW480细胞增殖速度明显加快，同时其Cyclin E1基因的