NF-kB反义核酸对胰腺癌细胞ICAM-1表达及侵袭转移能力的影响.pdfVIP

· 908· 肿瘤防治研究2007年第34卷第12期 NF—KB反义核酸对胰腺癌细胞ICAM一1 表达及侵袭转移能力的影响 储著凌 ，谢 敏 ，潘一明 ，朱海涛 Effect of NF-KB Antisense Oligodeoxynucleotides on Expression of ICAM-1 and Invasive A· bility of Pan creatic Carcinoma Cells CHU Zhu-ling ，XIE Min2，PAN Yi—minga，ZHU Hai—tao2 1．Department of General Surgery，454 Hospital of PLA，Nanjing 2101)02，China：2．Department of General Surgery，Drum Tower Hospital，Medical College of Nanjing University Abstract．Objective To investigate the effect of the nuclear factor-kappa B(NF—KB)subunit p65 anti— sense oligonucleotide(AS0DN)on the expression of intercellular adhesion molecule-1(ICA 1)and in— vasive ability of pancreatic carcinoma cells．Methods p65 ASODN were designed and constructed and were transfected into human pancreatic cancer eell line PANC 1．The rate of transfection was detected by flow cytometry and fluorescence microscope．Rrr_PCR was used to detect the expression levels of p65 and ICAM 1 mRNA．MTT assay and invasive tests were used to detect the proliferation and invasive ability of PANC-1 cells．Results p65 ASODN could be successfully transfected into PANC-1 cells．After trans— fection．the levels of p65 and ICAM_1 mRNA expression．cell proliferation and the invasive ability of PANC一1 cells were reduced(P【)．01)．Conclusion Transfection of p65 ASODN could effectively inhibit the expression of p65 mRNA and reduce invasive and metastasis ability of PANC-1 cells．The down-regu— lation of ICAM-1 may play an important role in the inhibitive effect of p65 ASODN． Key words：Antisense oligonucleotide；NF_KB；Pancreatic carcinoma；Intercellular adhesion molecule-1；In— vasion and metastasis 摘 要：目的 研究核因子一KB(NF-~B)亚单位p65反义寡核苷酸(ASODN)对人胰腺癌细胞细胞间粘附 分子一1(ICAM_1)表达及侵袭转移能力的影响。方法 设计合成p65 ASODN，体外转染人胰腺癌细胞 株PANC-1，用流式细胞仪和荧光显微镜检测转染效率，用RT-PCR检测p65及 ICAM-1 mR NA的表 达，用四甲基偶氮唑盐(MTT)法和细胞侵袭实验分析细胞增殖能力和侵袭转移能力的改变。结果 p65 ASODN可成功转染PANC一1细胞；转染后，p65及ICAM一1 mR NA表达明显减弱(P％0．01)；细胞 增殖活性和侵袭转移能力明显下降(P