游泳池水中嗜水气单胞菌的毒力因子的研究.pdfVIP

1016 中国卫生检验杂志2008年6月第l8卷第6期 Chinese Journal of Health Laboratory Technology，Jun 2008；Vol 18 No 6 【论著】 游泳池水中嗜水气单胞菌的毒力因子的研究 章乐怡，李毅，吴跃进，孙宝昌，陈慧燕，姜娴 (浙江省温州市疾病预防控制中心，浙江温州 325027) [摘要] 目的：对游泳池水检测出的19株嗜水气单胞菌进行毒力因子的研究。方法：通过蛋白酶试验检测嗜水气单胞 菌的蛋白酶，采用血平板法观察溶血素，采用PCR方法检测hlyA和aerA两种毒素基因，并进行小白鼠毒力试验。结果： 19株嗜水气单胞菌菌株含hlyA基因的占78．95％(15／19)，含aerA基因占73．68％(14／19)，18株嗜水气单胞菌菌株产 生蛋白酶，溶血试验显示的结果与hlyA基因检测的结果相符。菌株对小白鼠的平均致死率达85．96％(49／57)。结论： 游泳池水中分离出的嗜水气单胞菌大多携带毒力基因，采用多种方法检测其毒力因子能客观地对其毒力进行判定。 [关键词] 嗜水气单胞菌；毒力因子；小白鼠；致死性 [中图分类号] R378 [文献标识码] A [文章编号] 1004—8685(2008)06—1016—02 Investigation of virulent factors of aeromonas hydrophila in swimming pool water Zhang Le—yi，Li ，Wu Yue-jin，Sun Boo—chang，Chen Hui-yan，Jiang Xian (Wenzhou Center for Disease Control and Prevention，Wenzhou 325027，China) [Abstract] Objective：In the study，we investigated the virulence factors of 19 aeromonas hydrophila strains in swimming pool water．Methods：We detected the protease of aeromonas hydrophila by protease test，observed hemolysin using blood plate，detec— ted two toxin gene— hlyA and aerA by PCR method，and conducted mouse toxicity test．Results：In 19 aeromonas hydrophila strains those containing hlyA gene accounted for 78．95％ (t5／19)and aerA gene accounted for 73．68％ (14／19)，and 18 Aero— monas hydrophila strains produce protease．Hemolysis test is consistent with hlyA genetic testing in the results．Aeromonas hy— drophila strains resulted in that mouse death rate accounted for 85．96％ on the average(49／57)．Conclusion：The aeromonas hy— drophila isolated from the swimming pool water in most carries bacteria genes，SO we use various methods to detect its virulence factors． [Key words] Aeromonas hydrophila；Virulent；Factors mouse；Lethality 嗜水气单胞菌在引起散发性和爆发性腹泻中占重要位 溶蛋白圈判为阳性。 置。近年来由嗜水气单胞菌引起的食